APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
MCF-7 , Mouse heart
Predict Reactivity
Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 1% BSA, 20% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the center region of human DENR. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_11174217
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
density regulated re-initiation and release factor , DRP , DRP1 , SMAP-3
Background
This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq]
Database
Research Area
DATA IMAGES
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GTX115106 IHC-P Image
Immunohistochemical analysis of paraffin-embedded mouse muscle, using DENR(GTX115106) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115106 WB Image
Sample (30 ug of whole cell lysate)
A: MCF-7
12% SDS PAGE
GTX115106 diluted at 1:2000
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GTX115106 IHC-P Image
Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DENR(GTX115106) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX115106 ICC/IF Image
DENR antibody detects DENR protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DENR protein stained by DENR antibody (GTX115106) diluted at 1:500.
Blue: Hoechst 33342 staining.
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GTX115106 WB Image
Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX115106 diluted at 1:1000
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REFERENCE
There are currently no references for DENR antibody (GTX115106). Be the first to share your publications with this product.
REVIEW
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