GeneTex
United States (US)

DENR antibody

GTX115106_40681_IHC_18121410_767.jpg
GTX115106_40681_IFA_18121410_143.jpg
GTX115106_40681_WB_M_Heart_18121410_533.jpg
GTX115106_40681_IHC_M_18121410_464.jpg
GTX115106_40681_WB_18121410_153.jpg

Cat No. GTX115106

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

MCF-7 , mouse heart

Predict Reactivity

Rat, Bovine, Chicken, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DENR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Density Regulated Re-Initiation And Release Factor,Drp,Drp1,Smap-3,Denr

Background

This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq]

Database

DATA IMAGES
GTX115106_40681_IHC_18121410_767.jpg

GTX115106 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DENR(GTX115106) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115106_40681_IFA_18121410_143.jpg

GTX115106 ICC/IF Image

DENR antibody detects DENR protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DENR protein stained by DENR antibody (GTX115106) diluted at 1:500.
Blue: Hoechst 33342 staining.

GTX115106_40681_WB_M_Heart_18121410_533.jpg

GTX115106 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX115106 diluted at 1:1000

GTX115106_40681_IHC_M_18121410_464.jpg

GTX115106 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using DENR(GTX115106) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115106_40681_WB_18121410_153.jpg

GTX115106 WB Image

Sample (30 ug of whole cell lysate)

A: MCF-7

12% SDS PAGE

GTX115106 diluted at 1:2000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169