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DENR antibody

Anti-DENR antibody used in IHC (Paraffin sections) (IHC-P). GTX115106
Anti-DENR antibody used in IHC (Paraffin sections) (IHC-P). GTX115106
Anti-DENR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115106
Anti-DENR antibody used in Western Blot (WB). GTX115106
Anti-DENR antibody used in Western Blot (WB). GTX115106

Cat. No. GTX115106

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DENR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

22 kDa. ( Note )

Positive Control

MCF-7 , Mouse heart

Predict Reactivity

Rat, Bovine, Chicken, Xenopus tropicalis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DENR. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11174217

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

density regulated re-initiation and release factor , DRP , DRP1 , SMAP-3

Background

This gene encodes a protein whose expression was found to increase in cultured cells at high density but not during growth arrest. This gene was also shown to have increased expression in cells overexpressing HER-2/neu proto-oncogene. The protein contains an SUI1 domain. In budding yeast, SUI1 is a translation initiation factor that along with eIF-2 and the initiator tRNA-Met, directs the ribosome to the proper translation start site. Proteins similar to SUI have been found in mammals, insects, and plants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DENR antibody used in IHC (Paraffin sections) (IHC-P). GTX115106

GTX115106 IHC-P Image

Immunohistochemical analysis of paraffin-embedded mouse muscle, using DENR(GTX115106) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DENR antibody used in IHC (Paraffin sections) (IHC-P). GTX115106

GTX115106 IHC-P Image

Immunohistochemical analysis of paraffin-embedded HBL435 xenograft, using DENR(GTX115106) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DENR antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115106

GTX115106 ICC/IF Image

DENR antibody detects DENR protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DENR protein stained by DENR antibody (GTX115106) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DENR antibody used in Western Blot (WB). GTX115106

GTX115106 WB Image

Sample (30 ug of whole cell lysate)

A: MCF-7

12% SDS PAGE

GTX115106 diluted at 1:2000

Anti-DENR antibody used in Western Blot (WB). GTX115106

GTX115106 WB Image

Sample (50 ug of whole cell lysate)
A: mouse heart
12% SDS PAGE
GTX115106 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169