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DFFB antibody [36A349]

Anti-DFFB antibody [36A349] used in Western Blot (WB). GTX13592

Cat. No. GTX13592

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 36A349

Isotype

 IgG1

Application

 WB

Reactivity

 Human
Package
100 μg ($399)
See all DFFB products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:100 - 1:2000
Not tested in other applications.

Calculated MW

39 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Anti-DFFB monoclonal antibody was raised against a peptide corresponding to amino acids 205 to 222 of human DFFB.

Purification

Protein G purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA fragmentation factor subunit beta , CAD , CPAN , DFF-40 , DFF2 , DFF40

Cellular Localization

Cytoplasm,Nucleus

Background

Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DFFB antibody [36A349] used in Western Blot (WB). GTX13592

GTX13592 WB Image

WB analysis of HeLa cell lysate using GTX13592 CAD antibody [36A349].
Loading : 30μg

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399