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DFNA5 antibody

Anti-DFNA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03443
Anti-DFNA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03443
Anti-DFNA5 antibody used in Western Blot (WB). GTX03443
Anti-DFNA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03443
Anti-DFNA5 antibody used in Western Blot (WB). GTX03443

Cat. No. GTX03443

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human, Mouse, Rat
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:3000
ICC/IF 1:100-1:500
IHC-P 1:200
Not tested in other applications.

Calculated MW

55 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH 7.4, 150mM NaCl, 50% glycerol

Preservative

0.02% sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthesized peptide derived from human DFNA5(Accession O60443), corresponding to amino acid residues L416-P438.

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

gasdermin E , DFNA5 , ICERE-1

Cellular Localization

Cell membrane , Cytosol

Background

Hearing impairment is a heterogeneous condition with over 40 loci described. The protein encoded by this gene is expressed in fetal cochlea, however, its function is not known. Nonsyndromic hearing impairment is associated with a mutation in this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DFNA5 antibody used in IHC (Paraffin sections) (IHC-P). GTX03443

GTX03443 IHC-P Image

IHC-P analysis of rat liver tissue using GTX03443 DFNA5 antibody.
Antigen retrieval : Heat mediated antigen retrieval step in citrate buffer was performed.
Dilution : 1:100

Anti-DFNA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03443

GTX03443 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX03443 DFNA5 antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100
Dilution : 1:200

Anti-DFNA5 antibody used in Western Blot (WB). GTX03443

GTX03443 WB Image

WB analysis of Raw264.7 whole cell lysates using GTX03443 DFNA5 antibody.

Anti-DFNA5 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03443

GTX03443 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX03443 DFNA5 antibody.
Permeabilization : 0.1% Triton X-100
Red : Primary antibody
Green : beta tubulin
Blue : DAPI
Dilution : 1:200

Anti-DFNA5 antibody used in Western Blot (WB). GTX03443

GTX03443 WB Image

WB analysis of HeLa whole cell lysate using GTX03443 DFNA5 antibody. Lane 1 was treated with the blocking peptide.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399