APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 3μg/ml |
| 10ug/ml |
| 0.5-1ug/ml |
Not tested in other applications.
Calculated MW
Positive Control
The peptide used to generate this antibody is available for purchase (GTX89134-PEP).
Product Note
This antibody is not expected to cross-react with other DGAT members.
Predict Reactivity
Mouse, Rat, Dog(>80% identity)
PROPERTIES
Form
Liquid
Buffer
TBS, 0.5% BSA
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
0.50 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Peptide with sequence C-KLEHPTQQDIDLYH, from the internal region (near the C Terminus) of the protein sequence according to NP_115953.2.
Purification
Purified by ammonium sulphate precipitation followed by antigen affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
diacylglycerol O-acyltransferase 2 , ARAT , GS1999FULL , HMFN1045
Cellular Localization
Endoplasmic reticulum membrane
Background
This gene encodes one of two enzymes which catalyzes the final reaction in the synthesis of triglycerides in which diacylglycerol is covalently bound to long chain fatty acyl-CoAs. The encoded protein catalyzes this reaction at low concentrations of magnesium chloride while the other enzyme has high activity at high concentrations of magnesium chloride. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2011]
Database
Research Area
DATA IMAGES
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GTX89134 IHC-P Image
IHC-P analysis of human liver using GTX89134 DGAT2 antibody, Internal.
Antigen retrieval : Tris/EDTA buffer pH 9
Dilution : 3μg/ml
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GTX89134 ELISA Image
ELISA analysis of DGAT2 protein using GTX89134 DGAT2 antibody, Internal. This antibody was used as a detecting antibody.
Dilution : 0.5μg/ml
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GTX89134 FACS Image
FACS analysis of HeLa cells using GTX89134 DGAT2 antibody, Internal compared to an isotype control (black).
Dilution : 10 ug/ml
Permeabilization : 0.5% Triton
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REFERENCE
There are currently no references for DGAT2 antibody, Internal (GTX89134). Be the first to share your publications with this product.
REVIEW
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