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DGCR8 antibody

KO/KD
Anti-DGCR8 antibody used in Western Blot (WB). GTX130061
Anti-DGCR8 antibody used in Immunoprecipitation (IP). GTX130061
Anti-DGCR8 antibody used in Western Blot (WB). GTX130061

Cat. No. GTX130061

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP

Reactivity

 Human, Frog
Package
100 μl ($399),
25 μl ($169)
See all DGCR8 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IP 1:100-1:500
Not tested in other applications.

Calculated MW

86 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.15 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DGCR8. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2886166

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DGCR8 microprocessor complex subunit , C22orf12 , DGCRK6 , Gy1 , pasha

Background

This gene encodes a subunit of the microprocessor complex which mediates the biogenesis of microRNAs from the primary microRNA transcript. The encoded protein is a double-stranded RNA binding protein that functions as the non-catalytic subunit of the microprocessor complex. This protein is required for binding the double-stranded RNA substrate and facilitates cleavage of the RNA by the ribonuclease III protein, Drosha. Alternate splicing results in multiple transcript variants. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DGCR8 antibody used in Western Blot (WB). GTX130061

GTX130061 WB Image

KO/KD

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DGCR8 antibody (GTX130061) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DGCR8 antibody used in Immunoprecipitation (IP). GTX130061

GTX130061 IP Image

Immunoprecipitation of DGCR8 protein from Jurkat whole cell extracts using 5 μg of DGCR8 antibody (GTX130061).
Western blot analysis was performed using DGCR8 antibody (GTX130061).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Anti-DGCR8 antibody used in Western Blot (WB). GTX130061

GTX130061 WB Image

DGCR8 antibody detects DGCR8 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DGCR8 antibody (GTX130061) diluted at a dilution of 1:1000.

REFERENCE

Application Reference

Hadj-Moussa H et al. Cell Mol Life Sci 2018; (Epub) Micromanaging freeze tolerance: the biogenesis and regulation of neuroprotective microRNAs in frozen brains.
Application : WB
Reactivity : Frog
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169