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DGKA antibody

Anti-DGKA antibody used in IHC (Paraffin sections) (IHC-P). GTX55856
Anti-DGKA antibody used in Western Blot (WB). GTX55856
Anti-DGKA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55856

Cat. No. GTX55856

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($399)
See all DGKA products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:1000
ICC/IF 1:100 - 1:500
IHC-P 1:100 - 1:200
Not tested in other applications.

Calculated MW

83 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.42% Potassium Phosphate, 0.87% NaCl, 30% Glycerol

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the center region of DGKA. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diacylglycerol kinase alpha , DAGK , DAGK1 , DGK-alpha

Background

The protein encoded by this gene belongs to the eukaryotic diacylglycerol kinase family. It acts as a modulator that competes with protein kinase C for the second messenger diacylglycerol in intracellular signaling pathways. It also plays an important role in the resynthesis of phosphatidylinositols and phosphorylating diacylglycerol to phosphatidic acid. Several transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Apr 2017]

Database

Research Area

DATA IMAGES

Anti-DGKA antibody used in IHC (Paraffin sections) (IHC-P). GTX55856

GTX55856 IHC-P Image

IHC-P analysis of formalin fixed human brain tissue section using GTX55856 DGKA antibody.
Antigen retrieval : Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0)

Anti-DGKA antibody used in Western Blot (WB). GTX55856

GTX55856 WB Image

WB analysis of MDCK (A), COS7 (B) whole cell lysates using GTX55856 DGKA antibody.

Anti-DGKA antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55856

GTX55856 ICC/IF Image

ICC/IF analysis of formalin-fixed MDCK cells using GTX55856 DGKA antibody.
Red : Primary antibody
Blue : DAPI
Permeabilization : 0.1% Triton X-100 in TBS for 5-10 minutes

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399