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DGKG antibody [C1C3]

Anti-DGKG antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111375
Anti-DGKG antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111375
Anti-DGKG antibody [C1C3] used in Western Blot (WB). GTX111375

Cat. No. GTX111375

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DGKG products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

89 kDa. ( Note )

Positive Control

H1299

Predict Reactivity

Mouse, Rat(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.92 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DGKG. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950114

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diacylglycerol kinase gamma , DAGK3 , DGK-GAMMA

Cellular Localization

Cytoplasm , Membrane

Background

This gene encodes an enzyme that is a member of the type I subfamily of diacylglycerol kinases, which are involved in lipid metabolism. These enzymes generate phosphatidic acid by catalyzing the phosphorylation of diacylglycerol, a fundamental lipid second messenger that activates numerous proteins, including protein kinase C isoforms, Ras guanyl nucleotide-releasing proteins and some transient receptor potential channels. Diacylglycerol kinase gamma has been implicated in cell cycle regulation and in the negative regulation of macrophage differentiation in leukemia cells. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DGKG antibody [C1C3] used in IHC (Paraffin sections) (IHC-P). GTX111375

GTX111375 IHC-P Image

Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using DAG kinase gamma(GTX111375) antibody at 1:500 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DGKG antibody [C1C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX111375

GTX111375 ICC/IF Image

Immunofluorescence analysis of methanol-fixed HeLa, using DAG kinase gamma(GTX111375) antibody at 1:200 dilution.

Anti-DGKG antibody [C1C3] used in Western Blot (WB). GTX111375

GTX111375 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
7.5% SDS PAGE
GTX111375 diluted at 1:1000

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169