GeneTex
United States (US)

DGKI antibody

DGKI-antibody-GTX87789-ICC_IF-1_18122102_291.jpg
DGKI-antibody-GTX87789-WB-1_18122102_435.jpg

Cat No. GTX87789

Host Rabbit
Clonality Polyclonal
Application WB, ICC/IF, ELISA
Reactivity Human, Rat
APPLICATION

Application Note

We recommend the following starting dilutions:

WB: 1:500~1:1000
IF: 1:100~1:500
ELISA: 1:20000

Optimal dilutions should be determined experimentally by the end user.

Calculated MW

116 kDa. ( Note )

Specificity/Sensitivity

DGKI Antibody detects endogenous levels of total DGKI protein.
PROPERTIES

Form

Liquid

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human DGKI.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Diacylglycerol Kinase Iota,Dgk-Iota,Dgki

Background

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq]

Database

Research Area

DATA IMAGES
DGKI-antibody-GTX87789-ICC_IF-1_18122102_291.jpg

GTX87789 ICC/IF Image

Immunofluorescence analysis of HeLa cells, using DGKI Antibody. The picture on the right is treated with the synthesized peptide.

DGKI-antibody-GTX87789-WB-1_18122102_435.jpg

GTX87789 WB Image

Western blot analysis of extracts from 293 cells, using DGKI Antibody. The lane on the right is treated with the synthesized peptide.

Package List Price ($)
$ 339