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DGKI antibody

Anti-DGKI antibody used in Western Blot (WB). GTX87789
Anti-DGKI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87789

Cat. No. GTX87789

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μg ($399)
See all DGKI products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500~1:1000
ICC/IF 1:100~1:500
Not tested in other applications.

Calculated MW

117 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS (without Mg²⁺ and Ca²⁺) pH7.4, 150mM NaCl, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

The antiserum was produced against synthesized peptide derived from human DGKI (991-1040).

Purification

Purified by antigen-affinity chromatography
From serum

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DGKI , DGKIOTA , diacylglycerol kinase iota , DGK-ι

Cellular Localization

Cytoplasm,Nucleus

Background

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DGKI antibody used in Western Blot (WB). GTX87789

GTX87789 WB Image

WB analysis of 293 cell lysates using GTX87789 DGKI antibody. The lane on the right is blocked with the synthesized peptide.

Anti-DGKI antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX87789

GTX87789 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX87789 DGKI antibody. The picture on the right is blocked with the synthesized peptide.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399