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DHCR7 antibody [HL2764]

Recombinant antibody Orthogonal Overexpression
Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629
Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629

Cat. No. GTX639629

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL2764

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DHCR7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:10000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No Preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human DHCR7.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

7-dehydrocholesterol reductase , SLOS

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629

GTX639629 WB Image

Orthogonal

MCF-7 cytosol and membrane extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL2764] (GTX639629) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (Cyto:Cytosol; ME: membrane extract)

Anti-DHCR7 antibody [HL2764] used in Western Blot (WB). GTX639629

GTX639629 WB Image

Orthogonal Overexpression

Non-transfected (–) and transfected (+) Boiled and unboiled 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DHCR7 antibody [HL2764] (GTX639629) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
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Custom Conjugations