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DHCR7 antibody

Anti-DHCR7 antibody used in Western Blot (WB). GTX04343
Anti-DHCR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04343
Anti-DHCR7 antibody used in Western Blot (WB). GTX04343
Anti-DHCR7 antibody used in Western Blot (WB). GTX04343
Anti-DHCR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04343
Anti-DHCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX04343

Cat. No. GTX04343

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse
Package
400 μl ($399)
See all DHCR7 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:2000
ICC/IF 1:10-1:50
IHC-P 1:50-1:100
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.09% (W/V) sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

KLH conjugated synthetic peptide between 437-463 amino acids from the C-terminal region of human DHCR7.

Purification

Protein A purified followed by peptide affinity purification

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

7-dehydrocholesterol reductase , SLOS

Cellular Localization

Endoplasmic reticulum membrane

Background

This gene encodes an enzyme that removes the C(7-8) double bond in the B ring of sterols and catalyzes the conversion of 7-dehydrocholesterol to cholesterol. This gene is ubiquitously expressed and its transmembrane protein localizes to the endoplasmic reticulum membrane and nuclear outer membrane. Mutations in this gene cause Smith-Lemli-Opitz syndrome (SLOS); a syndrome that is metabolically characterized by reduced serum cholesterol levels and elevated serum 7-dehydrocholesterol levels and phenotypically characterized by cognitive disability, facial dysmorphism, syndactyly of second and third toes, and holoprosencephaly in severe cases to minimal physical abnormalities and near-normal intelligence in mild cases. Alternative splicing results in multiple transcript variants that encode the same protein.[provided by RefSeq, Aug 2009]

Database

Research Area

DATA IMAGES

Anti-DHCR7 antibody used in Western Blot (WB). GTX04343

GTX04343 WB Image

WB analysis of various sample lysates using GTX04343 DHCR7 antibody.
Dilution : 1:2000
Loading : 20 μg

Anti-DHCR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04343

GTX04343 ICC/IF Image

ICC/IF analysis of MCF7 cells using GTX04343 DHCR7 antibody.
Green : Primary antibody
Blue : DAPI

Anti-DHCR7 antibody used in Western Blot (WB). GTX04343

GTX04343 WB Image

WB analysis of mouse liver tissue lysate using GTX04343 DHCR7 antibody.
Dilution : 1:2000
Loading : 35 μg

Anti-DHCR7 antibody used in Western Blot (WB). GTX04343

GTX04343 WB Image

WB analysis of various sample lysates using GTX04343 DHCR7 antibody.
Dilution : 1:2000
Loading : 20 μg

Anti-DHCR7 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX04343

GTX04343 ICC/IF Image

ICC/IF analysis of 4% PFA fixed HeLa cells using GTX04343 DHCR7 antibody.
Green : Primary antibody
Blue : DAPI
Red : Phalloidin
Dilution : 1:25

Anti-DHCR7 antibody used in IHC (Paraffin sections) (IHC-P). GTX04343

GTX04343 IHC-P Image

IHC-P analysis of human testis tissue using GTX04343 DHCR7 antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399