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DHFR antibody [6G7]

Overexpression
Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617
Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617
Anti-DHFR antibody [6G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84617
Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617
Anti-DHFR antibody [6G7] used in Western Blot (WB). GTX84617
Anti-DHFR antibody [6G7] used in Western Blot (WB). GTX84617
Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617

Cat. No. GTX84617

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6G7

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Dog, Monkey
Package
100 μl ($489)
See all DHFR products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500
ICC/IF Assay dependent
IHC-P Assay dependent
Not tested in other applications.

Calculated MW

21 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full-length protein expressed in 293T cell transfected with human DHFR expression vector

Purification

Purified by affinity chromatography
From tissue culture supernatant

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrofolate reductase , DHFRP1 , DYR

Cellular Localization

Mitochondrion,Cytoplasm

Background

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis.

Database

Research Area

DATA IMAGES

Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617

GTX84617 IHC-P Image

IHC-P analysis of human ovary adenocarcinoma tissue using GTX84617 DHFR antibody [6G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617

GTX84617 IHC-P Image

IHC-P analysis of breast adenocarcinoma tissue using GTX84617 DHFR antibody [6G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-DHFR antibody [6G7] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX84617

GTX84617 ICC/IF Image

ICC/IF analysis of COS7 cells transiently transfected with DHFR plasmid using GTX84617 DHFR antibody [6G7].

Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617

GTX84617 IHC-P Image

IHC-P analysis of thyroid carcinoma tissue using GTX84617 DHFR antibody [6G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

Anti-DHFR antibody [6G7] used in Western Blot (WB). GTX84617

GTX84617 WB Image

Overexpression

WB analysis of HEK293T cells transfected with DHFR plasmid (Right) or empty vector (Left) for 48 hrs using GTX84617 DHFR antibody [6G7].
Loading : 5 ug per lane

Anti-DHFR antibody [6G7] used in Western Blot (WB). GTX84617

GTX84617 WB Image

WB analysis of various cell lines using GTX84617 DHFR antibody [6G7].
Loading : 35 ug per lane

Anti-DHFR antibody [6G7] used in IHC (Paraffin sections) (IHC-P). GTX84617

GTX84617 IHC-P Image

IHC-P analysis of kidney carcinoma tissue using GTX84617 DHFR antibody [6G7].
Antigen retrieval : Heat-induced epitope retrieval by 10mM citrate buffer, pH6.0, 100ºC for 10min.
Dilution : 1:50

REFERENCE

Application Reference

Wang SA et al. Mol Oncol 2023; NCI677397 targeting USP24-mediated induction of lipid peroxidation induces ferroptosis in drug-resistant cancer cells
Application : WB
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 489