Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using DHFR(GTX117705) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunofluorescence analysis of paraformaldehyde-fixed A431, using DHFR(GTX117705) antibody at 1:200 dilution.

DHFR antibody detects DHFR protein by western blot analysis.
A. 30 μg PC-12 whole cell lysate/extract
12% SDS-PAGE
DHFR antibody (GTX117705) dilution: 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Whole cell extract (30 μg) was separated by 12% SDS-PAGE, and the membrane was blotted with DHFR antibody (GTX117705) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunoprecipitation of DHFR protein from 293T whole cell extracts using 5 μg of DHFR antibody (GTX117705).
Western blot analysis was performed using DHFR antibody (GTX117705).
EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.