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DHRS2 antibody

KO/KD
Anti-DHRS2 antibody used in Western Blot (WB). GTX123431
Anti-DHRS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123431

Cat. No. GTX123431

Reference 1
1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DHRS2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HepG2

Product Note

Knockdown/Knockout validation was supported by references (PMID:29632240)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DHRS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11173747

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dehydrogenase/reductase 2 , HEP27 , SDR25C1

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES

Anti-DHRS2 antibody used in Western Blot (WB). GTX123431

GTX123431 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX123431 diluted at 1:3000

Anti-DHRS2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX123431

GTX123431 ICC/IF Image

DHRS2 antibody detects DHRS2 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: DHRS2 protein stained by DHRS2 antibody (GTX123431) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

Application Reference

Oda T et al. J Cell Sci 2018; pii : jcs.210724 Acute HSF1 depletion induces cellular senescence through the MDM2-p53-p21 pathway in human diploid fibroblasts.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

DHRS2 antibody Cat. No. GTX123431

Rating
( Average 5 based on 1 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
Anti-DHRS2 antibody used in Western Blot (WB). GTX123431
Date : Anonymous submitted on 04-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 5-10 μg
Blocking : 0.3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Lane Description : Lane 1: control-shRNA transfected, Lane 2: shDHRS2 transfected
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169