GeneTex
United States (US)

DHRS2 antibody

GTX123431_40639_WB_18121410_515.jpg
GTX123431_40639_20141205_IFA_18121410_669.jpg

Cat No. GTX123431

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

HepG2

Specificity/Sensitivity

Knockdown/Knockout validation was supported by references (PMID:29632240)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DHRS2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dehydrogenase/Reductase 2,Hep27,Sdr25C1,Dhrs2

Cellular Localization

Nucleus

Database

Research Area

DATA IMAGES
GTX123431_40639_WB_18121410_515.jpg

GTX123431 WB Image

Sample (30 ug of whole cell lysate)
A: HepG2
12% SDS PAGE
GTX123431 diluted at 1:3000

GTX123431_40639_20141205_IFA_18121410_669.jpg

GTX123431 ICC/IF Image

DHRS2 antibody detects DHRS2 protein at mitochondria by immunofluorescent analysis.
Sample: Hep G2 cells were fixed in 2% paraformaldehyde/culture medium at 37oC for 30 min.
Green: DHRS2 protein stained by DHRS2 antibody (GTX123431) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE
REVIEW

DHRS2 antibody Cat No. GTX123431

Rating ( Average 5 based on 1 users reviews)
Application
Western Blot(WB) ( Average 5 based on 1 users reviews)
Date : Anonymous submitted on 04-May-2018
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF7 cells
Amount used : 5-10 μg
Blocking : 0.3% skim milk, 25°C, 0.5Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Lane Description : Lane 1: control-shRNA transfected, Lane 2: shDHRS2 transfected
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169