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DHX30 antibody, N-term

Anti-DHX30 antibody, N-term used in Western Blot (WB). GTX47513
Anti-DHX30 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47513

Cat. No. GTX47513

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, IHC-P

Reactivity

Human
Package
50 μg ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.2-2.5 ug/ml
IHC-P 2-10 ug/ml
Not tested in other applications.

Calculated MW

134 kDa. ( Note )

Positive Control

MCF-7

PROPERTIES

Form

Liquid

Buffer

PBS, 2% Sucrose

Preservative

0.09% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5-1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

A synthetic peptide corresponding to a N-terminal region of Human DHX30

Purification

Affinity Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DExH-box helicase 30 , DDX30 , NEDMIAL , RETCOR

Cellular Localization

Cytoplasm,Mitochondrion

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this DEAD box protein family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The family member encoded by this gene is a mitochondrial nucleoid protein that associates with mitochondrial DNA. It has also been identified as a component of a transcriptional repressor complex that functions in retinal development, and it is required to optimize the function of the zinc-finger antiviral protein. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Feb 2013]

Database

Research Area

DATA IMAGES

Anti-DHX30 antibody, N-term used in Western Blot (WB). GTX47513

GTX47513 WB Image

WB analysis of MCF-7 cells using GTX47513 DHX30 antibody at 0.2-1μg/ml.

Anti-DHX30 antibody, N-term used in IHC (Paraffin sections) (IHC-P). GTX47513

GTX47513 IHC-P Image

IHC-P analysis of human heart tissue using GTX47513 DHX30 antibody at 1:100. Left to right : DAPI, DHX30, Merge. Low pH, heat-induced antigen retrieval method utilizing Sodium Citrate buffer was performed.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399