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DHX9 antibody

Anti-DHX9 antibody used in IHC (Paraffin sections) (IHC-P). GTX130971
Anti-DHX9 antibody used in IHC (Paraffin sections) (IHC-P). GTX130971
Anti-DHX9 antibody used in Western Blot (WB). GTX130971
Anti-DHX9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130971

Cat No. GTX130971

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P

Reactivity

Human
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

HeLa , HeLa nuclear extract

Predict Reactivity

Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.35 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DHX9. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

DExH-box helicase 9 , DDX9 , LKP , NDH2 , NDHII , RHA

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]

Database

DATA IMAGES

Anti-DHX9 antibody used in IHC (Paraffin sections) (IHC-P). GTX130971

GTX130971 IHC-P Image

DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in CAL 27 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded CAL 27 xenograft.
DHX9 antibody (GTX130971) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DHX9 antibody used in IHC (Paraffin sections) (IHC-P). GTX130971

GTX130971 IHC-P Image

DHX9 antibody detects DHX9 protein at cytoplasm and nucleus in A549 xenograft by immunohistochemical analysis.
Sample: Paraffin-embedded A549 xenograft.
DHX9 antibody (GTX130971) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DHX9 antibody used in Western Blot (WB). GTX130971

GTX130971 WB Image

DHX9 antibody detects DHX9 protein by western blot analysis. HeLa whole cell extracts and nuclear extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DHX9 antibody (GTX130971) diluted at 1:1000.

Anti-DHX9 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX130971

GTX130971 ICC/IF Image

DHX9 antibody detects DHX9 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DHX9 protein stained by DHX9 antibody (GTX130971) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 μm.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 329
$ 169