GeneTex
United States (US)

DIAPH1 antibody [C2C3], C-term

GTX102057_39609_IHC_M_19011506_804.jpg
GTX102057_WB_19011506_640.jpg
GTX102057_39609_IHC_19011506_240.jpg
GTX102057_39609_IFA_19011506_932.jpg

Cat No. GTX102057

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

Positive Control

H1299 , Raji

Predict Reactivity

Rat, Pig(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DIAPH1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DFNA1 antibody, DIA1 antibody, DRF1 antibody, FLJ25265 antibody, LFHL1 antibody, hDIA1 antibody, DIAPH1 antibody, protein diaphanous homolog 1 antibody, diaphanous-related formin-1 antibody, diaphanous-related formin 1 antibody, diaphanous homolog 1 (Drosophila) antibody

Cellular Localization

Cell membrane , Cell projection , ruffle membrane , Cytoplasm , cytoskeleton

Background

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX102057_39609_IHC_M_19011506_804.jpg

GTX102057 IHC-P Image

DIAPH1 antibody [C2C3], C-term detects DIAPH1 protein at membrane on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
DIAPH1 antibody [C2C3], C-term (GTX102057) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102057_WB_19011506_640.jpg

GTX102057 WB Image

Sample (30 ug of whole cell lysate)
A: H1299
B: Raji
5% SDS PAGE
GTX102057 diluted at 1:1000

GTX102057_39609_IHC_19011506_240.jpg

GTX102057 IHC-P Image

DIAPH1 antibody [C2C3], C-term detects DIAPH1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
DIAPH1 antibody [C2C3], C-term (GTX102057) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX102057_39609_IFA_19011506_932.jpg

GTX102057 ICC/IF Image

Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DIAPH1(GTX102057) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169