APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
H1299 , Raji
Predict Reactivity
Rat, Pig(>80% identity)
PROPERTIES
Form
Liquid
Buffer
PBS, 40% Glycerol
Preservative
0.01% Thimerosal
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human DIAPH1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_1240705
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
diaphanous related formin 1 , DFNA1 , DIA1 , DRF1 , LFHL1 , SCBMS , hDIA1
Cellular Localization
Cell membrane , Cell projection , ruffle membrane , Cytoplasm , cytoskeleton
Background
This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq]
Database
Research Area
DATA IMAGES
![Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057](/templates/default/js/lightbox/images/loading.gif "Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057")
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GTX102057 IHC-P Image
DIAPH1 antibody [C2C3], C-term detects DIAPH1 protein at cytoplasm on human colon carcinoma by immunohistochemical analysis.
Sample: Paraffin-embedded colon carcinoma.
DIAPH1 antibody [C2C3], C-term (GTX102057) dilution: 1:250.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102057 IHC-P Image
DIAPH1 antibody [C2C3], C-term detects DIAPH1 protein at membrane on mouse lung by immunohistochemical analysis.
Sample: Paraffin-embedded mouse lung.
DIAPH1 antibody [C2C3], C-term (GTX102057) dilution: 1:500.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min
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GTX102057 WB Image
Sample (30 ug of whole cell lysate)
A: H1299
B: Raji
5% SDS PAGE
GTX102057 diluted at 1:1000
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GTX102057 ICC/IF Image
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using DIAPH1(GTX102057) antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with GTX11304 (Red) at 1:2000.
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GTX102057 WB Image
Various whole cell extracts (30 μg) were separated by 5% SDS-PAGE, and the membrane was blotted with DIAPH1 antibody [C2C3], C-term (GTX102057) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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REFERENCE
There are currently no references for DIAPH1 antibody [C2C3], C-term (GTX102057). Be the first to share your publications with this product.
REVIEW
There are currently no reviews for DIAPH1 antibody [C2C3], C-term (GTX102057). Be the first to share your experience with this product.
![Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057](/upload/website/prouct_img/small/GTX102057/GTX102057_39609_IHC_w_23060100_401.webp "Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057](/upload/website/prouct_img/small/GTX102057/GTX102057_39609_IHC_M_w_23060100_595.webp "Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057](/upload/website/prouct_img/small/GTX102057/GTX102057_WB_w_23060100_581.webp "Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102057](/upload/website/prouct_img/small/GTX102057/GTX102057_39609_IFA_w_23060100_352.webp "Anti-DIAPH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057](/upload/website/prouct_img/small/GTX102057/GTX102057_39609_20240216_WB_TPM_watermark_24021917_643.webp "Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057](/upload/website/prouct_img/normal/GTX102057/GTX102057_39609_IHC_w_23060100_401.webp "Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057](/upload/website/prouct_img/normal/GTX102057/GTX102057_39609_IHC_M_w_23060100_595.webp "Anti-DIAPH1 antibody [C2C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057](/upload/website/prouct_img/normal/GTX102057/GTX102057_WB_w_23060100_581.webp "Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102057](/upload/website/prouct_img/normal/GTX102057/GTX102057_39609_IFA_w_23060100_352.webp "Anti-DIAPH1 antibody [C2C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX102057")
![Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057](/upload/website/prouct_img/normal/GTX102057/GTX102057_39609_20240216_WB_TPM_watermark_24021917_643.webp "Anti-DIAPH1 antibody [C2C3], C-term used in Western Blot (WB). GTX102057")