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DIAPH1 antibody

Anti-DIAPH1 antibody used in Western Blot (WB). GTX33153
Anti-DIAPH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33153

Cat. No. GTX33153

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Mouse
Package
100 μl ($399)
See all DIAPH1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:100
Not tested in other applications.

Calculated MW

141 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1149-1263 of human DIAPH1 (NP_001073280.1).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diaphanous related formin 1 , DFNA1 , DIA1 , DRF1 , LFHL1 , SCBMS , hDIA1

Cellular Localization

Cell membrane,Cytoplasm, cytoskeleton,Cytoplasm,Nucleus

Background

This gene is a homolog of the Drosophila diaphanous gene, and has been linked to autosomal dominant, fully penetrant, nonsyndromic sensorineural progressive low-frequency hearing loss. Actin polymerization involves proteins known to interact with diaphanous protein in Drosophila and mouse. It has therefore been speculated that this gene may have a role in the regulation of actin polymerization in hair cells of the inner ear. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DIAPH1 antibody used in Western Blot (WB). GTX33153

GTX33153 WB Image

WB analysis of various sample lysates using GTX33153 DIAPH1 antibody.
Dilution : 1:1000
Loading : 25μg per lane

Anti-DIAPH1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX33153

GTX33153 ICC/IF Image

ICC/IF analysis of U2OS cells using GTX33153 DIAPH1 antibody.
Blue : DAPI

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399