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DIAPH2 antibody

Anti-DIAPH2 antibody used in Western Blot (WB). GTX10562
Anti-DIAPH2 antibody used in Western Blot (WB). GTX10562

Cat. No. GTX10562

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IP, ELISA

Reactivity

 Human
Package
50 μg ($399)
See all DIAPH2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:1000-1:6000
IP 1:100
ELISA 1:12000-1:60000
Not tested in other applications.

Calculated MW

126 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

20mM Potassium Phosphate, 150mM NaCl

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.4 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide corresponding to amino acid residues at the C-Terminus of Human DIA-2.

Purification

Purified by antigen-affinity chromatography.
From serum

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

diaphanous related formin 2 , DIA , DIA2 , DRF2 , POF , POF2 , POF2A

Background

This gene may play a role in the development and normal function of the ovaries. Mutations of this gene have been linked to premature ovarian failure. Alternative splicing results in two protein isoforms. Furthermore, each splice variant undergoes additional splicing in the 3' UTR. Overall 4 splice variants have been described.

Database

Research Area

DATA IMAGES

Anti-DIAPH2 antibody used in Western Blot (WB). GTX10562

GTX10562 WB Image

Western blot using GeneTex Affinity Purified anti-DIAPH2 antibody (GTX10562) shows detection of a 132-kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

Anti-DIAPH2 antibody used in Western Blot (WB). GTX10562

GTX10562 WB Image

WB analysis of HEK293 whole cell lysate using GTX10562 DIAPH2 antibody.
Loading : 20 μg
Dilution : 1:500

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 399