WB analysis of HEK293 whole cell lysate using GTX10562 DIAPH2 antibody.
Loading : 20 μg
Dilution : 1:500

Western blot using GeneTex Affinity Purified anti-DIAPH2 antibody (GTX10562) shows detection of a 132-kDa band corresponding to DIAPH2 in a lysate prepared from human derived HEK293 cells. Approximately 20 ug of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-DIA-2 antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.