GeneTex
United States (US)

DIAPH3 antibody [C1C3]

GTX102892_39728_CT_IHC_19011506_445.jpg
GTX102892_39728_20161013_WB_M_liver_19011506_872.jpg

Cat No. GTX102892

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

137 kDa. ( Note )

Positive Control

mouse liver

Predict Reactivity

Rat, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DKFZp434C0931 antibody, DKFZp686A13178 antibody, DRF3 antibody, FLJ34705 antibody, diap3 antibody, mDia2 antibody, DIAPH3 antibody, diaphanous-related formin-3 antibody, diaphanous-related formin 3 antibody, "auditory neuropathy, autosomal dominant 1 antibody", protein diaphanous homolog 3 antibody, diaphanous homolog 3 (Drosophila) antibody

Background

Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
Show More +

Database

DATA IMAGES
GTX102892_39728_CT_IHC_19011506_445.jpg

GTX102892 IHC-P Image

DIAPH3 antibody [C1C3] detects DIAPH3 protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
DIAPH3 antibody [C1C3] (GTX102892) dilution: 1:100.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

GTX102892_39728_20161013_WB_M_liver_19011506_872.jpg

GTX102892 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody [C1C3] (GTX102892) diluted at 1:500.

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169