*Optimal dilutions/concentrations should be determined by the researcher.
Not tested in other applications.
Rat, Bovine(>80% identity)
0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Recombinant protein encompassing a sequence within the center region of human DIAPH3. The exact sequence is proprietary.
Purified by antigen-affinity chromatography.
For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
DKFZp434C0931 antibody, DKFZp686A13178 antibody, DRF3 antibody, FLJ34705 antibody, diap3 antibody, mDia2 antibody, DIAPH3 antibody, diaphanous-related formin-3 antibody, diaphanous-related formin 3 antibody, "auditory neuropathy, autosomal dominant 1 antibody", protein diaphanous homolog 3 antibody, diaphanous homolog 3 (Drosophila) antibody
Binds to GTP-bound form of Rho and to profilin. Acts in a Rho-dependent manner to recruit profilin to the membrane, where it promotes actin polymerization. It is required for cytokinesis, stress fiber formation, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics.
GTX102892 IHC-P Image
DIAPH3 antibody [C1C3] detects DIAPH3 protein at cytoplasm on human placenta by immunohistochemical analysis.
Sample: Paraffin-embedded placenta.
DIAPH3 antibody [C1C3] (GTX102892) dilution: 1:100.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
GTX102892 WB Image
Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DIAPH3 antibody [C1C3] (GTX102892) diluted at 1:500.