GeneTex
United States (US)

DIS3 antibody

GTX115645_41213_IHC_R_18121410_910.jpg
GTX115645_41213_IHC_M_18121410_619.jpg
GTX115645_41213_WB_18121410_277.jpg
GTX115645_41213_IFA_18121410_495.jpg

Cat No. GTX115645

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

91 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , IMR-32 , SK-N-AS
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human DIS3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dis3 Homolog, Exosome Endoribonuclease And 3'-5' Exoribonuclease,2810028N01Rik,Exosc11,Kiaa1008,Rrp44,Dis3P,Dis3

Cellular Localization

Cytoplasm , Nucleus , nucleolus

Background

Component of the exosome 3'->5' exoribonuclease complex. Required for the 3'-processing of the 7S pre-RNA to the mature nuclear complex. Also associated with the GTPase Ran. Has a 3'-5' exonuclease activity.

Database

Research Area

DATA IMAGES
GTX115645_41213_IHC_R_18121410_910.jpg

GTX115645 IHC-P Image

DIS3 antibody detects DIS3 protein at cytosol and nucleus on rat hind brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat hind brain.
DIS3 antibody (GTX115645) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115645_41213_IHC_M_18121410_619.jpg

GTX115645 IHC-P Image

DIS3 antibody detects DIS3 protein at cytosol and nucleus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DIS3 antibody (GTX115645) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX115645_41213_WB_18121410_277.jpg

GTX115645 WB Image

DIS3 antibody detects DIS3 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DIS3 antibody (GTX115645) diluted by 1:1000.

GTX115645_41213_IFA_18121410_495.jpg

GTX115645 ICC/IF Image

DIS3 antibody detects DIS3 protein at nucleus by immunofluorescent analysis.
Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DIS3 protein stained by DIS3 antibody (GTX115645) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169