APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
Application |
Dilution |
1:500 - 1:2000 |
1:50 - 1:200 |
1:50 - 1:200 |
Not tested in other applications.
Calculated MW
PROPERTIES
Form
Liquid
Buffer
PBS pH7.3, 0.02% sodium azide, 50% glycerol.
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Antigen Species
Human
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 245-514 of human DKC1 (NP_001354.1).
Purification
Purified by affinity chromatography
Conjugation
Unconjugated
Note
For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
Synonyms
dyskerin pseudouridine synthase 1 , CBF5 , DKC , DKCX , NAP57 , NOLA4 , XAP101
Background
This gene functions in two distinct complexes. It plays an active role in telomerase stabilization and maintenance, as well as recognition of snoRNAs containing H/ACA sequences which provides stability during biogenesis and assembly into H/ACA small nucleolar RNA ribonucleoproteins (snoRNPs). This gene is highly conserved and widely expressed, and may play additional roles in nucleo-cytoplasmic shuttling, DNA damage response, and cell adhesion. Mutations have been associated with X-linked dyskeratosis congenita. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
Database
DATA IMAGES
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GTX32562 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32562 DKC1 antibody. Dilution : 1:200
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GTX32562 IHC-P Image
IHC-P analysis of human esophageal cancer tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human esophagus tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 WB Image
WB analysis of various samples using GTX32562 DKC1 antibody. Dilution : 1:1000 Loading : 25μg per lane
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GTX32562 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human kidney tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human colon tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human liver cancer tissue using GTX32562 DKC1 antibody. Dilution : 1:200
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GTX32562 ICC/IF Image
ICC/IF analysis of U2OS cell using GTX32562 DKC1 antibody. Blue : DAPI
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GTX32562 IHC-P Image
IHC-P analysis of mouse pancreas tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human gastric cancer tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of mouse kidney tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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GTX32562 IHC-P Image
IHC-P analysis of human stomach tissue using GTX32562 DKC1 antibody. Dilution : 1:200
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GTX32562 IHC-P Image
IHC-P analysis of mouse brain tissue using GTX32562 DKC1 antibody. Dilution : 1:200
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GTX32562 IHC-P Image
IHC-P analysis of human stomach tissue using GTX32562 DKC1 antibody. Dilution : 1:100
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REFERENCE
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REVIEW
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