DKK1 antibody, C-term

Cat. No. GTX89683

Host	Goat
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, IHC-P, FACS
Reactivity	Human
Package	100 μg ($399)

See all DKK1 products

Datasheet
Document

View product citations for antibody GTX89683 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	1-3μg/ml
ICC/IF	Assay dependent
IHC-P	10μg/ml
FACS	Assay dependent

Not tested in other applications.

Calculated MW

29 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX89683-PEP).

Predict Reactivity

Rat, Bovine, Dog, Pig(>80% identity)

PROPERTIES

Form

Liquid

Buffer

TBS, 0.5% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.50 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Peptide with sequence CDHHQASNSSRLHT, from the C Terminus of the protein sequence according to NP_036374.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dickkopf WNT signaling pathway inhibitor 1 , DKK-1 , SK

Cellular Localization

Secreted

Background

This gene encodes a member of the dickkopf family of proteins. Members of this family are secreted proteins characterized by two cysteine-rich domains that mediate protein-protein interactions. The encoded protein binds to the LRP6 co-receptor and inhibits beta-catenin-dependent Wnt signaling. This gene plays a role in embryonic development and may be important in bone formation in adults. Elevated expression of this gene has been observed in numerous human cancers and this protein may promote proliferation, invasion and growth in cancer cell lines. [provided by RefSeq, Sep 2017]

Database

Gene ID: 22943 DKK1
UniProt: O94907 DKK1

Research Area

DATA IMAGES

GTX89683 IHC-P Image

IHC-P analysis of human placenta using GTX89683 DKK1 antibody, C-term.
Antigen retrieval : citrate buffer pH 6
Dilution : 10μg/ml

GTX89683 WB Image

WB analysis of MCF7 whole cell lysate using GTX89683 DKK1 antibody, C-term.
Dilution : 1μg/ml
Loading : 35μg protein in RIPA buffer

GTX89683 FACS Image

FACS analysis of PFA-fixed HepG2 cells using GTX89683 DKK1 antibody, C-term.
Blue line : Primary antibody
Black line : Unimmunized Goat IgG
Permeabilization : 0.5% Triton
Dilution : 10μg/ml

GTX89683 ICC/IF Image

ICC/IF analysis of PFA-fixed HepG2 cells using GTX89683 DKK1 antibody, C-term.
Green : Primary antibody
Blue : DAPI
Negative control : Unimmunized Goat IgG
Permeabilization : 0.15% Triton
Dilution : 10μg/ml

REFERENCE

There are currently no references for DKK1 antibody, C-term (GTX89683). Be the first to share your publications with this product.

Submit a Reference

REVIEW

There are currently no reviews for DKK1 antibody, C-term (GTX89683). Be the first to share your experience with this product.

Submit a Review

SDS
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty