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DKK3 antibody [8A5C6]

Anti-DKK3 antibody [8A5C6] used in Western Blot (WB). GTX60665
Anti-DKK3 antibody [8A5C6] used in IHC (Paraffin sections) (IHC-P). GTX60665
Anti-DKK3 antibody [8A5C6] used in ELISA (ELISA). GTX60665

Cat. No. GTX60665

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 8A5C6

Isotype

 IgG1

Application

 WB, IHC-P, FACS, ELISA

Reactivity

 Human
Package
100 μg ($409)
See all DKK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
IHC-P 1/200 - 1/1000
FACS 1/200 - 1/400
ELISA 1/10000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

0.05% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant fragment of human DKK3 (AA: 91-350 ) expressed in E. Coli.

Purification

Protein G Purified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dickkopf WNT signaling pathway inhibitor 3 , REIC , RIG

Cellular Localization

Secreted

Background

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DKK3 antibody [8A5C6] used in Western Blot (WB). GTX60665

GTX60665 WB Image

WB analysis of HEK293 (1) and DKK3 (AA: 91-350)-hIgGFc transfected HEK293 (2) cell lysate using GTX60665 DKK3 antibody [8A5C6].

Anti-DKK3 antibody [8A5C6] used in IHC (Paraffin sections) (IHC-P). GTX60665

GTX60665 IHC-P Image

IHC-P analysis of human brain tissue using GTX60665 DKK3 antibody [8A5C6].

Anti-DKK3 antibody [8A5C6] used in ELISA (ELISA). GTX60665

GTX60665 ELISA Image

ELISA analysis of antigen using GTX60665 DKK3 antibody [8A5C6].
Black : Control antigen 100ng
Purple : Antigen 10ng
Blue : Antigen 50ng
Red : Antigen 100ng

REFERENCE

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REVIEW

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SDS
PBS.pdf
Sodium Azide.pdf
Package List Price ($)
$ 409