GeneTex
United States (US)

DKK3 antibody [N1C3]

GTX100571_39471_20181227_ICC_IF_R_19022001_819.jpg
GTX100571_39471_20141128_IHC_19011506_859.jpg
GTX100571_WB_19011506_655.jpg
GTX100571_39471_WB_M_brain_19011506_830.jpg

Cat No. GTX100571

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

A549 , mouse brain

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DKK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dickkopf Wnt Signaling Pathway Inhibitor 3,Reic,Rig,Dkk3

Cellular Localization

Secreted

Background

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX100571_39471_20181227_ICC_IF_R_19022001_819.jpg

GTX100571 ICC/IF Image

DKK3 antibody [N1C3] detects DKK3 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DKK3 stained by DKK3 antibody [N1C3] (GTX100571) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX100571_39471_20141128_IHC_19011506_859.jpg

GTX100571 IHC-P Image

DKK3 antibody [N1C3] detects DKK3 protein at cytoplasm on human normal liver by immunohistochemical analysis.
Sample: Paraffin-embedded human normal liver.
DKK3 antibody [N1C3] (GTX100571) diluted at 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX100571_WB_19011506_655.jpg

GTX100571 WB Image

Sample (30 ug of whole cell lysate)
A: A549
10% SDS PAGE
GTX100571 diluted at 1:1000

GTX100571_39471_WB_M_brain_19011506_830.jpg

GTX100571 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX100571 diluted at 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169