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DKK3 antibody [N2C3]

Anti-DKK3 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115842
Anti-DKK3 antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115842
Anti-DKK3 antibody [N2C3] used in Western Blot (WB). GTX115842

Cat. No. GTX115842

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T

Predict Reactivity

Bovine, Dog, Pig, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DKK3. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11164615

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dickkopf WNT signaling pathway inhibitor 3 , REIC , RIG

Cellular Localization

Secreted

Background

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DKK3 antibody [N2C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX115842

GTX115842 ICC/IF Image

DKK3 antibody [N2C3] detects DKK3 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DKK3 stained by DKK3 antibody [N2C3] (GTX115842) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DKK3 antibody [N2C3] used in IHC (Frozen sections) (IHC-Fr). GTX115842

GTX115842 IHC-Fr Image

DKK3 antibody [N2C3] detects DKK3 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebral cortex.
Green: DKK3 stained by DKK3 antibody [N2C3] (GTX115842) diluted at 1:250.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DKK3 antibody [N2C3] used in Western Blot (WB). GTX115842

GTX115842 WB Image

Sample (30 ug of whole cell lysate)
A: 293T
10% SDS PAGE
GTX115842 diluted at 1:1000

REFERENCE

There are currently no references for DKK3 antibody [N2C3] (GTX115842). Be the first to share your publications with this product.

REVIEW

There are currently no reviews for DKK3 antibody [N2C3] (GTX115842). Be the first to share your experience with this product.
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169