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DKK3 antibody

Anti-DKK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX55588
Anti-DKK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55588
Anti-DKK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX55588
Anti-DKK3 antibody used in Western Blot (WB). GTX55588

Cat. No. GTX55588

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Mouse, Rat
Package
100 μl ($399)
See all DKK3 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500 - 1:2000
ICC/IF 1:50 - 1:200
IHC-P 1:50 - 1:200
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 50% Glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 22-350 of human DKK3 (NP_037385.2).

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dickkopf WNT signaling pathway inhibitor 3 , REIC , RIG

Cellular Localization

Secreted

Background

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DKK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX55588

GTX55588 IHC-P Image

IHC-P analysis of mouse heart tissue using GTX55588 DKK3 antibody.
Dilution : 1:100

Anti-DKK3 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX55588

GTX55588 ICC/IF Image

ICC/IF analysis of NIH/3T3 cells using GTX55588 DKK3 antibody.
Blue : DAPI
Dilution : 1:100

Anti-DKK3 antibody used in IHC (Paraffin sections) (IHC-P). GTX55588

GTX55588 IHC-P Image

IHC-P analysis of rat heart tissue using GTX55588 DKK3 antibody.
Dilution : 1:100

Anti-DKK3 antibody used in Western Blot (WB). GTX55588

GTX55588 WB Image

WB analysis of mouse brain tissue lysate using GTX55588 DKK3 antibody.
Dilution : 1:1000
Loading : 25μg per lane

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399