GeneTex
United States (US)

DLD antibody

KO/KD
GTX101245_39506_20181102_WB_KO_watermark_19011519_539.jpg
GTX101245_WB_19011506_611.jpg
GTX101245_39511_WB_M_Brain_19011506_619.jpg
GTX101245_39506_20151231_WB_R_19011506_587.jpg
GTX101245_39511_IHC_19011506_902.jpg
GTX101245_39506_20170913_IFA_19011506_858.jpg

Cat No. GTX101245

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

293T , mouse brain , PC-12 , Rat2

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DLD. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dihydrolipoamide Dehydrogenase,Dldd,Dldh,E3,Gcsl,Lad,Phe3,Dld

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101245_39506_20181102_WB_KO_watermark_19011519_539.jpg

GTX101245 WB Image

KO/KD

Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody (GTX101245) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
CRISPR knockout cell lines generated by www.edigene.com

GTX101245_WB_19011506_611.jpg

GTX101245 WB Image

Sample(30 μg of whole cell lysate)
A:293T
10% SDS PAGE
GTX101245 diluted at 1:1000

GTX101245_39511_WB_M_Brain_19011506_619.jpg

GTX101245 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101245 diluted at 1:1000

GTX101245_39506_20151231_WB_R_19011506_587.jpg

GTX101245 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody (GTX101245) diluted at 1:1000.

GTX101245_39511_IHC_19011506_902.jpg

GTX101245 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DLD(GTX101245) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101245_39506_20170913_IFA_19011506_858.jpg

GTX101245 ICC/IF Image

DLD antibody detects DLD protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DLD protein stained by DLD antibody (GTX101245) diluted at 1:500.
Blue: Hoechst 33342 staining.

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169