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DLD antibody

KO/KD
Anti-DLD antibody used in Western Blot (WB). GTX101245
Anti-DLD antibody used in IHC (Paraffin sections) (IHC-P). GTX101245
Anti-DLD antibody used in Western Blot (WB). GTX101245
Anti-DLD antibody used in Western Blot (WB). GTX101245
Anti-DLD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101245
Anti-DLD antibody used in Western Blot (WB). GTX101245

Cat. No. GTX101245

Reference 3
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DLD products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

54 kDa. ( Note )

Positive Control

Mouse brain , 293T , PC-12 , Rat2 , HeLa

Predict Reactivity

Bovine, Dog, Chicken, Pig, Xenopus laevis(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DLD. The exact sequence is proprietary.

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

RRID

AB_1240715

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrolipoamide dehydrogenase , DLDD , DLDH , E3 , GCSL , LAD , PHE3

Cellular Localization

Mitochondrion matrix

Background

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DLD antibody used in Western Blot (WB). GTX101245

GTX101245 WB Image

KO/KD

Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody (GTX101245) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DLD antibody used in IHC (Paraffin sections) (IHC-P). GTX101245

GTX101245 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human gastric cancer, using DLD(GTX101245) antibody at 1:100 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DLD antibody used in Western Blot (WB). GTX101245

GTX101245 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody (GTX101245) diluted at 1:1000.

Anti-DLD antibody used in Western Blot (WB). GTX101245

GTX101245 WB Image

Sample (50 ug of whole cell lysate)
A: Mouse brain
10% SDS PAGE
GTX101245 diluted at 1:1000

Anti-DLD antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX101245

GTX101245 ICC/IF Image

DLD antibody detects DLD protein at mitochondria by immunofluorescent analysis.
Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.
Green: DLD protein stained by DLD antibody (GTX101245) diluted at 1:500.
Blue: Hoechst 33342 staining.
Anti-DLD antibody used in Western Blot (WB). GTX101245

GTX101245 WB Image

Sample(30 μg of whole cell lysate)
A:293T
10% SDS PAGE
GTX101245 diluted at 1:1000

REFERENCE

Application Reference

Ogawa T et al. Physiol Rep 2023; 11 (4) : e15608 Downregulation of extramitochondrial BCKDH and its uncoupling from AMP deaminase in type 2 diabetic OLETF rat hearts.
Application : WB
Reactivity : Rat
Green SR et al. Biochim Biophys Acta Proteins Proteom 2020; 1868 (9) : 140448 Regulation of the α-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardson's ground squirrel (Urocitellus richardsonii).
Application : WB
Reactivity : Human
Shin D et al. Redox Biol 2020; 30 : 101418 Dihydrolipoamide dehydrogenase regulates cystine deprivation-induced ferroptosis in head and neck cancer.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

DLD antibody Cat. No. GTX101245

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 2 users reviews)
Anti-DLD antibody used in Western Blot (WB). GTX101245
Date : Anonymous submitted on 11-Mar-2024
Score :
Application Tested : WB
Sample Species : Hu
Sample : MCF10A and MCF7 cells
Amount used : 10μg
Blocking : 0.5% skim milk in TBS, 25°C, 1Hr
Primary Antibody : 1:2000, 4°C, 12Hr
Anti-DLD antibody used in Western Blot (WB). GTX101245
Date : Anonymous submitted on 30-Jul-2020
Score :
Application Tested : WB
Sample Species : Hu
Sample : Jurkat cells
Amount used : 5 μg
Blocking : 5% milk in TBST, 4°C, 16Hr
Primary Antibody : 1:1000, 25°C, 1Hr
Submit a Review
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169