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DLGAP1 antibody

Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264
Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264
Anti-DLGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133264
Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264
Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264
Anti-DLGAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133264

Cat No. GTX133264

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF, IHC-P, IHC-Fr
Reactivity Mouse, Rat
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

110 kDa. ( Note )

Positive Control

mouse hippocampus , mouse brain , rat brain

Predict Reactivity

Human, Bovine(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1.87 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse DLGAP1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

DLG associated protein 1,4933422O14Rik,9630002F18,AI845682,AI848168,BB075781,D17Bwg0511e,GKAP/SAPAP,GKPA/SAPAP,Gkap,Sapap1,mKIAA4162

Cellular Localization

Cell membrane; Peripheral membrane protein , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse , Cell membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse

Background

Part of the postsynaptic scaffold in neuronal cells.

Database

Research Area

DATA IMAGES
Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264

GTX133264 IHC-P Image

DLGAP1 antibody detects DLGAP1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
DLGAP1 antibody (GTX133264) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264

GTX133264 WB Image

Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DLGAP1 antibody (GTX133264) diluted at 1:3000.
The observed M.W. is based on the publication: PMID: 21041448

Anti-DLGAP1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX133264

GTX133264 ICC/IF Image

DLGAP1 antibody detects DLGAP1 protein by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DLGAP1 stained by DLGAP1 antibody (GTX133264) diluted at 1:500.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DLGAP1 antibody used in Western Blot (WB). GTX133264

GTX133264 WB Image

Mouse tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLGAP1 antibody (GTX133264) diluted at 1:3000.

Anti-DLGAP1 antibody used in IHC (Paraffin sections) (IHC-P). GTX133264

GTX133264 IHC-P Image

DLGAP1 antibody detects DLGAP1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DLGAP1 antibody (GTX133264) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DLGAP1 antibody used in IHC (Frozen sections) (IHC-Fr). GTX133264

GTX133264 IHC-Fr Image

DLGAP1 antibody detects DLGAP1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: DLGAP1 stained by DLGAP1 antibody (GTX133264) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 10 min

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169