APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 1:500-1:3000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
| 1:100-1:1000 |
Not tested in other applications.
Calculated MW
Positive Control
mouse brain , rat brain , mouse hippocampus
Predict Reactivity
Human, Bovine(>80% identity)
PROPERTIES
Form
Liquid
Buffer
1XPBS (pH7), 20% Glycerol
Preservative
0.025% ProClin 300
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1.87 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Mouse
Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of mouse DLGAP1. The exact sequence is proprietary.
Purification
Purified by antigen-affinity chromatography.
Conjugation
Unconjugated
RRID
AB_2886897
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DLG associated protein 1 , 4933422O14Rik , 9630002F18 , AI845682 , AI848168 , BB075781 , D17Bwg0511e , GKAP/SAPAP , GKPA/SAPAP , Gkap , Sapap1 , mKIAA4162
Cellular Localization
Cell membrane; Peripheral membrane protein , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse , Cell membrane , Cell junction , synapse , postsynaptic cell membrane , postsynaptic density , synapse
Background
Part of the postsynaptic scaffold in neuronal cells.
Database
Research Area
DATA IMAGES
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GTX133264 ICC/IF Image
DLGAP1 antibody detects DLGAP1 protein by immunofluorescent analysis.Sample: DIV9 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: DLGAP1 stained by DLGAP1 antibody (GTX133264) diluted at 1:500.Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.Blue: Fluoroshield with DAPI (GTX30920).
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GTX133264 IHC-Fr Image
DLGAP1 antibody detects DLGAP1 Protein expression by immunohistochemical analysis.
Sample: Frozen-sectioned adult mouse cerebellum.
Green: DLGAP1 stained by DLGAP1 antibody (GTX133264) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Antigen Retrieval: Citrate buffer, pH 6.0, 10 min
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GTX133264 IHC-P Image
DLGAP1 antibody detects DLGAP1 protein at cytoplasm in rat brain by immunohistochemical analysis.
Sample: Paraffin-embedded rat brain.
DLGAP1 antibody (GTX133264) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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GTX133264 WB Image
Mouse tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLGAP1 antibody (GTX133264) diluted at 1:3000.
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GTX133264 WB Image
Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DLGAP1 antibody (GTX133264) diluted at 1:3000.
The observed M.W. is based on the publication: PMID: 21041448
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GTX133264 IHC-P Image
DLGAP1 antibody detects DLGAP1 protein at cytoplasm in mouse brain by immunohistochemical analysis.
Sample: Paraffin-embedded mouse brain.
DLGAP1 antibody (GTX133264) diluted at 1:500.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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REFERENCE
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REVIEW
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