APPLICATION
Application Note
*Optimal dilutions/concentrations should be determined by the researcher.
| Application |
Recommended Dilution |
| 0.5 - 1 μg/mL |
| 5 μg/mL |
| Assay dependent |
Not tested in other applications.
Calculated MW
Positive Control
Human Brain Tissue Slide(GTX22203) , Rat Brain Tissue Lysate(GTX27942)
Product Note
At least three isoforms are known to exist. This antibody will not cross-react with other SAPAP proteins.
PROPERTIES
Form
Liquid
Buffer
PBS
Preservative
0.02% Sodium azide
Storage
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Concentration
1 mg/ml (Please refer to the vial label for the specific concentration.)
Antigen Species
Human
Immunogen
DLGAP1 antibody was raised against a 18 amino acid synthetic peptide from near the center of human DLGAP1.The immunogen is located within amino acids 640 - 690 of DLGAP1.
Purification
Purified by antigen-affinity chromatography
Conjugation
Unconjugated
Note
For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.
TARGET
Synonyms
DLG associated protein 1 , DAP-1 , DAP-1-ALPHA , DAP-1-BETA , DAP1 , DLGAP1A , DLGAP1B , GKAP , SAPAP1
Cellular Localization
Cell membrane,Cell junction, synapse
Background
SAP90/PSD-95-associated protein 1 (SAPAP1, also known as DLGAP1 or GKAP) is a member of a protein family whose members specifically interact with PSD-95/SAP90, a membrane-associated guanylate kinase localized at postsynaptic density (PSD) in neuronal cells. Like the other SAPAP proteins, SAPAP1 is thought to be an adaptor protein that also interacts with different synaptic scaffolding proteins, cytoskeletal and signaling components, such as focal adhesion kinase (FAK) and proline-rich tyrosine kinase 2 (PYK2). SAPAP1 mRNA is targeted to cell bodies in a similar manner to SAPAP2 and -4, whereas SAPAP3 mRNA is detected mainly in cell bodies. SAPAP1 protein however, is targeted to the synapse and is not reliant on the synaptic localization of PSD-95 or the synaptic scaffolding molecule (S-SCAM). At least three isoforms of SAPAP1 are known to exist. This SAPAP1 antibody will not cross-react with other SAPAP proteins.
Database
Research Area
DATA IMAGES
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GTX85396 IHC-P Image
IHC-P analysis of human brain tissue using GTX85396 DLGAP1 antibody.
Working concentration : 5 μg/ml
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GTX85396 WB Image
WB analysis of rat brain tissue lysate using GTX85396 DLGAP1 antibody.
Working concentration : (A) 0.5 and (B) 1 μg/ml
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GTX85396 IHC-P Image
IHC-P analysis of human brain tissue using GTX85396 DLGAP1 antibody.
Working concentration : 20 μg/ml
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REFERENCE
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REVIEW
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