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DLK/MAP3K12 antibody

Orthogonal
Anti-DLK/MAP3K12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Anti-DLK/MAP3K12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124127

Cat. No. GTX124127

Reference 9
2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P, IHC-Fr

Reactivity

 Human, Mouse, Rat
Package
100 μl ($399),
25 μl ($169)
See all DLK/MAP3K12 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

Helicobacter pylori

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse DLK/MAP3K12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_11170703

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

mitogen-activated protein kinase kinase kinase 12 , DLK , MUK , Zpk

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Database

Research Area

DATA IMAGES

Anti-DLK/MAP3K12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124127

GTX124127 IHC-Fr Image

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody (GTX124127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

Sample (50 μg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX124127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein by western blot analysis. Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DLK/MAP3K12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124127

GTX124127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAP3K12(GTX124127) antibody at 1:250 dilution.

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

Rat tissue extract (50 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted at 1:1000.

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

DLK/MAP3K12 antibody detects MAP3K12 protein by Western blot analysis.
A. 20 μg mouse embryonic stem cell whole cell lysate/extract
7.5 % SDS-PAGE
DLK/MAP3K12 antibody (GTX124127) dilution: 1:1000

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

Various tissue extracts (50 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

Orthogonal

The data was published in the journal Elife in 2019.PMID: 31042147

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

Orthogonal

The data was published in the journal Elife in 2019.PMID: 31042147

Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127

GTX124127 WB Image

Orthogonal

The data was published in the journal Mol Neurodegener in 2019. PMID: 31775817

Anti-DLK/MAP3K12 antibody used in IHC (Frozen sections) (IHC-Fr). GTX124127

GTX124127 IHC-Fr Image

Orthogonal

The data was published in the journal Mol Neurodegener in 2019. PMID: 31775817

REFERENCE

Application Reference

Duncan GJ et al. bioRxiv 2023; Remyelination protects neurons from DLK-mediated neurodegeneration.
K?ster KA et al. Naunyn Schmiedebergs Arch Pharmacol 2023; 396 (6) : 1223-1233 Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.
Lindborg JA et al. Cell Rep 2021; 34 (9) : 108777 Optic nerve regeneration screen identifies multiple genes restricting adult neural repair.
Application : WB
Augert A et al. Cancer Cell 2020; 38 (1) : 97-114.e7 MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.
Application : WB
Reactivity : Mouse
Tenenbaum M et al. Cell Mol Life Sci 2020; The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development.
Reactivity : Rat
Show More +
Submit a Reference

REVIEW

DLK/MAP3K12 antibody Cat. No. GTX124127

Rating
( Average 5 based on 2 users reviews)
Application
Western Blot(WB)
( Average 5 based on 1 users reviews)
IHC (Paraffin sections)(IHC-P)
( Average 5 based on 1 users reviews)
Anti-DLK/MAP3K12 antibody used in Western Blot (WB). GTX124127
Date : Anonymous submitted on 20-Dec-2022
Score :
Application Tested : WB
Sample Species : Ms
Sample : mouse neural tissue
Amount used : 10ug
Blocking : BSA, 25°C, 2Hr
Primary Antibody : 1:1000, 4°C, 18Hr
Anti-DLK/MAP3K12 antibody used in IHC (Paraffin sections) (IHC-P). GTX124127
Date : Anonymous submitted on 17-Nov-2022
Score :
Application Tested : IHC-P
Sample Species : Ms
Sample : brain-injured mouse (after 3days of injury)
Blocking : 0.5% skim milk in PBST, RT°C, 2Hr
Fixation : 4% PFA
Permeabilization /Antigen retrieval : PBS containing Triton X-100 (PBST)
Primary Antibody : 1:1000, 4°C, 18Hr
Submit a Review
Application Reference
Duncan GJ et al. bioRxiv 2023; Remyelination protects neurons from DLK-mediated neurodegeneration.
K?ster KA et al. Naunyn Schmiedebergs Arch Pharmacol 2023; 396 (6):1223-1233 Increase of c-FOS promoter transcriptional activity by the dual leucine zipper kinase.
Lindborg JA et al. Cell Rep 2021; 34 (9):108777 Optic nerve regeneration screen identifies multiple genes restricting adult neural repair.
Application : WB
Augert A et al. Cancer Cell 2020; 38 (1):97-114.e7 MAX Functions as a Tumor Suppressor and Rewires Metabolism in Small Cell Lung Cancer.
Application : WB
Reactivity : Mouse
Tenenbaum M et al. Cell Mol Life Sci 2020; The Map3k12 (Dlk)/JNK3 signaling pathway is required for pancreatic beta-cell proliferation during postnatal development.
Reactivity : Rat
Welsbie DS et al. Mol Neurodegener 2019; 14 (1):44 Targeted disruption of dual leucine zipper kinase and leucine zipper kinase promotes neuronal survival in a model of diffuse traumatic brain injury.
Application : IHC-P
Reactivity : Mouse
Wang G et al. Elife 2019; 8 Structural plasticity of actin-spectrin membrane skeleton and functional role of actin and spectrin in axon degeneration.
Application : WB
Reactivity : Mouse
Larhammar M et al. J Neurosci 2017; (Epub) The Ste20 family kinases MAP4K4, MINK1 and TNIK, converge to regulate stress induced JNK signaling in neurons.
Application : WB
Reactivity : Mouse
Wu CC et al. Cell Cycle 2015; 14 (8):1207-17 Akt suppresses DLK for maintaining self-renewal of mouse embryonic stem cells.
Application : WB
Reactivity : Mouse
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169