GeneTex
United States (US)

DLK/MAP3K12 antibody

GTX124127_40702_20160921_IHC-Fr_18121410_470.jpg
GTX124127_40611_20141107_WB_M_18121410_387.jpg
GTX124127_40611_IHC_18121410_143.jpg
GTX124127_40653_20160901_WB_R_brain_18121410_703.jpg
GTX124127_40611_WB_M_Brain_18121410_621.jpg
GTX124127_40653_WB_M_ESC_18121410_440.jpg

Cat No. GTX124127

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

96 kDa. ( Note )

Positive Control

Neuro 2A , C8D30 , NIH-3T3 , Raw264.7 , C2C12 , Mouse brain , mouse embryonic stem cell , rat brain
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Mouse

Immunogen

Recombinant protein encompassing a sequence within the center region of mouse DLK/MAP3K12. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Mitogen-Activated Protein Kinase Kinase Kinase 12,Dlk,Muk,Zpk,Map3K12

Cellular Localization

Cytoplasm , Membrane; Peripheral membrane protein

Background

May be an activator of the JNK/SAPK pathway. Phosphorylates beta-casein, histone 1 and myelin basic protein in vitro.

Database

Research Area

DATA IMAGES
GTX124127_40702_20160921_IHC-Fr_18121410_470.jpg

GTX124127 IHC-Fr Image

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein expression by immunohistochemical analysis.
Sample: Frozen sectioned E13.5 Rat brain.
Green: DLK/MAP3K12 protein stained by DLK/MAP3K12 antibody (GTX124127) diluted at 1:250.
Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

GTX124127_40611_20141107_WB_M_18121410_387.jpg

GTX124127 WB Image

DLK/MAP3K12 antibody detects DLK/MAP3K12 protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted by 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124127_40611_IHC_18121410_143.jpg

GTX124127 IHC-P Image

Immunohistochemical analysis of paraffin-embedded human breast cancer, using MAP3K12(GTX124127) antibody at 1:250 dilution.

GTX124127_40653_20160901_WB_R_brain_18121410_703.jpg

GTX124127 WB Image

Rat tissue extract (50 µg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DLK/MAP3K12 antibody (GTX124127) diluted at 1:1000.

GTX124127_40611_WB_M_Brain_18121410_621.jpg

GTX124127 WB Image

Sample (50 µg of whole cell lysate)
A: mouse brain
7.5% SDS PAGE
GTX124127 diluted at 1:1000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX124127_40653_WB_M_ESC_18121410_440.jpg

GTX124127 WB Image

DLK/MAP3K12 antibody detects MAP3K12 protein by Western blot analysis.
A. 20 μg mouse embryonic stem cell whole cell lysate/extract
7.5 % SDS-PAGE
DLK/MAP3K12 antibody (GTX124127) dilution: 1:1000

REFERENCE
SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169