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DLL1 antibody

Anti-DLL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03469
Anti-DLL1 antibody used in Flow cytometry (FACS). GTX03469
Anti-DLL1 antibody used in Western Blot (WB). GTX03469
Anti-DLL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03469

Cat. No. GTX03469

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, FACS, ELISA

Reactivity

Human, Mouse, Rat, Monkey
Package
100 μg ($429)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 0.1-0.25μg/ml
ICC/IF 4μg/ml
IHC-P 0.5-1μg/ml
FACS 1-3μg/1x10⁶ cells
ELISA 0.1-0.5μg/ml
Not tested in other applications.

Calculated MW

78 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

4mg Trehalose, 0.9mg NaCl, 0.2mg Na₂HPO₄

Preservative

0.01mg sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.5 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

E.coli-derived human DLL1 recombinant protein (Position: Q18-K239).

Purification

Purified by antigen-affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

delta like canonical Notch ligand 1 , DELTA1 , DL1 , Delta

Cellular Localization

Apical cell membrane

Background

DLL1 is a human homolog of the Notch Delta ligand and is a member of the delta/serrate/jagged family. It plays a role in mediating cell fate decisions during hematopoiesis. It may play a role in cell-to-cell communication. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DLL1 antibody used in IHC (Paraffin sections) (IHC-P). GTX03469

GTX03469 IHC-P Image

IHC-P analysis of human placenta tissue using GTX03469 DLL1 antibody.
Antigen retireval : Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution).
Dilution : 1 μg/ml

Anti-DLL1 antibody used in Flow cytometry (FACS). GTX03469

GTX03469 FACS Image

FACS analysis of THP-1 cells using GTX03469 DLL1 antibody.
Blue : Primary antibody
Green : Isotype control
Red : Unlabelled cell
Antibody amount : 1μg/1x10⁶ cells

Anti-DLL1 antibody used in Western Blot (WB). GTX03469

GTX03469 WB Image

WB analysis of various sample lysates using GTX03469 DLL1 antibody.
Lane 1 : HeLa whole cell lysate
Lane 2 : COS-7 whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : Raji whole cell lysate
Lane 5 : SW620 whole cell lysate
Lane 6 : HepG2 whole cell lysate
Lane 7 : U2OS whole cell lysate
Lane 8 : rat heart tissue lysate
Lane 9 : C6 whole cell lysate
Lane 10 : mouse heart tissue lysate
Lane 11 : Neuro-2a whole cell lysate
Lane 12 : RAW264.7 whole cell lysate
Dilution : 0.25 μg/ml
Loading : 50μg per lane

Anti-DLL1 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX03469

GTX03469 ICC/IF Image

ICC/IF analysis of A549 cells using GTX03469 DLL1 antibody.
Antigen retireval : Enzyme antigen retrieval was performed using enzyme antigen retrieval reagent for 15 mins.
Blue : DAPI
Dilution : 4μg/mL

REFERENCE

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REVIEW

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Package List Price ($)
$ 429