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DLST antibody [N3C3]

Anti-DLST antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112555
Anti-DLST antibody [N3C3] used in Western Blot (WB). GTX112555
Anti-DLST antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112555
Anti-DLST antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112555
Anti-DLST antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112555
Anti-DLST antibody [N3C3] used in Western Blot (WB). GTX112555

Cat. No. GTX112555

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human, Rat, Zebrafish
Package
100 μl ($399),
25 μl ($169)
See all DLST products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

49 kDa. ( Note )

Predict Reactivity

Mouse, Bovine, Chicken, Pig, Xenopus laevis, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.7 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DLST. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036769

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

dihydrolipoamide S-succinyltransferase , DLTS

Cellular Localization

Mitochondrion

Background

The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components: 2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and lipoamide dehydrogenase (E3).

Database

Research Area

DATA IMAGES

Anti-DLST antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112555

GTX112555 IHC-P Image

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using DLST antibody [N3C3] (GTX112555) at 1:300 dilution.

Anti-DLST antibody [N3C3] used in Western Blot (WB). GTX112555

GTX112555 WB Image

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX112555 diluted at 1:1000

Anti-DLST antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112555

GTX112555 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DLST(GTX112555) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DLST antibody [N3C3] used in IHC (Paraffin sections) (IHC-P). GTX112555

GTX112555 IHC-P Image

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using DLST(GTX112555) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DLST antibody [N3C3] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX112555

GTX112555 ICC/IF Image

Immunofluorescence analysis of methanol-fixed A431, using DLST(GTX112555) antibody at 1:500 dilution.

Anti-DLST antibody [N3C3] used in Western Blot (WB). GTX112555

GTX112555 WB Image

Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLST antibody [N3C3] (GTX112555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

Application Reference

Green SR et al. Biochim Biophys Acta Proteins Proteom 2020; 1868 (9) : 140448 Regulation of the α-ketoglutarate dehydrogenasecomplex during hibernation in a small mammal, the Richardson's ground squirrel (Urocitellus richardsonii).
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169