Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with DLST antibody [N3C3] (GTX112555) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Immunofluorescence analysis of methanol-fixed A431, using DLST(GTX112555) antibody at 1:500 dilution.

Immunohistochemical analysis of paraffin-embedded RT2 xenograft, using DLST(GTX112555) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using DLST(GTX112555) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 μg of whole cell lysate)
A: zebrafish eye
10% SDS PAGE
GTX112555 diluted at 1:1000

Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using DLST antibody [N3C3] (GTX112555) at 1:300 dilution.