GeneTex
United States (US)

DLX1 antibody [N1C3-2]

GTX108804_40660_WB_19011507_168.jpg
GTX108804_40660_20170922_WB_M_R_19011507_930.jpg
GTX108804_40660_20180125_IHC-Fr_M_19011507_884.jpg

Cat No. GTX108804

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-Fr
Reactivity Human, Mouse, Rat
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-Fr 1:100-1:1000
Not tested in other applications.

Calculated MW

27 kDa. ( Note )

Positive Control

U87-MG , mouse brain , rat brain

Predict Reactivity

Bovine, Chicken, Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Full length human DLX1 Recombinant protein.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Distal-Less Homeobox 1,Dlx1

Cellular Localization

Nucleus

Background

This gene encodes a member of a homeobox transcription factor gene family similiar to the Drosophila distal-less gene. The encoded protein is localized to the nucleus where it may function as a transcriptional regulator of signals from multiple TGF-{beta} superfamily members. The encoded protein may play a role in the control of craniofacial patterning and the differentiation and survival of inhibitory neurons in the forebrain. This gene is located in a tail-to-tail configuration with another member of the family on the long arm of chromosome 2. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq]

Database

DATA IMAGES
GTX108804_40660_WB_19011507_168.jpg

GTX108804 WB Image

Sample (30 µg of whole cell lysate)
A: U87-MG
12% SDS PAGE
GTX108804 diluted at 1:3000
The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108804_40660_20170922_WB_M_R_19011507_930.jpg

GTX108804 WB Image

Various tissue extracts (50 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with Dlx1 antibody [N1C3-2] (GTX108804) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

GTX108804_40660_20180125_IHC-Fr_M_19011507_884.jpg

GTX108804 IHC-Fr Image

Dlx1 antibody [N1C3-2] detects Dlx1 protein by immunohistochemical analysis.
Sample: Frozen-sectioned mouse cerebellum.
Green: Dlx1 stained by Dlx1 antibody [N1C3-2] (GTX108804) diluted at 1:250.
Red: NF-H, stained by NF-H antibody [GT114] (GTX634289) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169