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DLX2 antibody [HL1942]

Recombinant antibody Orthogonal Overexpression
Anti-DLX2 antibody [HL1942] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637779
Anti-DLX2 antibody [HL1942] used in Western Blot (WB). GTX637779
Anti-DLX2 antibody [HL1942] used in Western Blot (WB). GTX637779

Cat. No. GTX637779

Host

 Rabbit

Clonality

 Monoclonal

Clone Name

 HL1942

Isotype

 IgG

Application

 WB, ICC/IF

Reactivity

 Human, Rat
Package
100 μl ($399),
25 μl ($169)
See all DLX2 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Predict Reactivity

Mouse, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant fragment of human DLX2

Purification

Affinity purified by Protein A.

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

distal-less homeobox 2 , TES-1 , TES1

Cellular Localization

Nucleus

Background

Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 2. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DLX2 antibody [HL1942] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX637779

GTX637779 ICC/IF Image

DLX2 antibody [HL1942] detects DLX2 protein at nucleus by immunofluorescent analysis.
Sample: DIV9 rat E18 primary cortical neuron and glia cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DLX2 stained by DLX2 antibody [HL1942] (GTX637779) diluted at 1:250.
Red: Tau, an axon marker, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DLX2 antibody [HL1942] used in Western Blot (WB). GTX637779

GTX637779 WB Image

Orthogonal

293T whole cell and nuclear extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DLX2 antibody [HL1942] (GTX637779) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. (WCE: whole cell extract; NE: nuclear extract)

Anti-DLX2 antibody [HL1942] used in Western Blot (WB). GTX637779

GTX637779 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 12% SDS-PAGE, and the membrane was blotted with DLX2 antibody [HL1942] (GTX637779) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

REFERENCE

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REVIEW

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Package List Price ($)
$ 399
$ 169
Custom Conjugations