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DLX2 antibody

Anti-DLX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129123
Anti-DLX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129123
Anti-DLX2 antibody used in Western Blot (WB). GTX129123

Cat No. GTX129123

Host

Rabbit

Clonality

Polyclonal

Isotype

IgG

Application

WB, ICC/IF, IHC-P, IHC-Fr

Reactivity

Human, Mouse, Rat
Package
100 μl ($329),
25 μl ($169)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:5000-1:20000
ICC/IF Assay dependent
IHC-P 1:100-1:1000
IHC-Fr Assay dependent
Not tested in other applications.

Calculated MW

34 kDa. ( Note )

Positive Control

human DLX2-transfected 293T

Predict Reactivity

Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DLX2. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

distal-less homeobox 2 , TES-1 , TES1

Cellular Localization

Nucleus

Background

Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. The DLX proteins are postulated to play a role in forebrain and craniofacial development. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 2. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DLX2 antibody used in IHC (Paraffin sections) (IHC-P). GTX129123

GTX129123 IHC-P Image

DLX2 antibody detects DLX2 protein by immunohistochemical analysis.
Samples: Paraffin-Embedded mouse retina.
Green: DLX2 protein stained by DLX2 antibody (GTX129123) diluted at 1:250.
Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody [GT1338] (GTX631831) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DLX2 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX129123

GTX129123 ICC/IF Image

DLX2 antibody detects DLX2 protein by immunofluorescent analysis.
Sample: DIV10 rat E18 primary cortical neuron cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DLX2 stained by DLX2 antibody (GTX129123) diluted at 1:500.
Red: Tau, stained by Tau antibody [GT287] (GTX634809) diluted at 1:500.
Blue: Fluoroshield with DAPI (GTX30920).

Anti-DLX2 antibody used in Western Blot (WB). GTX129123

GTX129123 WB Image

DLX2 antibody detects DLX2 protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg whole cell lysate/extract of human DLX2-transfected 293T cells
12 % SDS-PAGE
DLX2 antibody (GTX129123) dilution: 1:20000

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169