Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DMC1 antibody [N1C1] (GTX103062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using DMC1(GTX103062) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX103062 diluted at 1:1000

DMC1 antibody [N1C1] detects DMC1 protein at nucleolus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DMC1 antibody [N1C1] (GTX103062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min