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DMC1 antibody [N1C1]

Anti-DMC1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103062
Anti-DMC1 antibody [N1C1] used in Western Blot (WB). GTX103062
Anti-DMC1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103062
Anti-DMC1 antibody [N1C1] used in Western Blot (WB). GTX103062

Cat. No. GTX103062

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, IHC-P

Reactivity

 Human, Mouse
Package
100 μl ($399),
25 μl ($169)
See all DMC1 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

Molt-4 , mouse testis

Predict Reactivity

Rat, Zebrafish, Bovine, Japanese Medaka(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DMC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_2036772

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA meiotic recombinase 1 , DMC1H , LIM15 , dJ199H16.1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is essential for meiotic homologous recombination. Genetic recombination in meiosis plays an important role in generating diversity of genetic information. The product of this gene is structurally and evolutionary related to the products of the yeast RAD51 and E. coli RecA genes. Alternative splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DMC1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103062

GTX103062 IHC-P Image

DMC1 antibody [N1C1] detects DMC1 protein at nucleolus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DMC1 antibody [N1C1] (GTX103062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DMC1 antibody [N1C1] used in Western Blot (WB). GTX103062

GTX103062 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX103062 diluted at 1:1000

Anti-DMC1 antibody [N1C1] used in IHC (Paraffin sections) (IHC-P). GTX103062

GTX103062 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using DMC1(GTX103062) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DMC1 antibody [N1C1] used in Western Blot (WB). GTX103062

GTX103062 WB Image

Mouse tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with DMC1 antibody [N1C1] (GTX103062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

REFERENCE

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REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169