GeneTex
United States (US)

DMC1 antibody [N1C1]

GTX103062_IHC_19011506_513.jpg
GTX103062_WB_19011506_440.jpg
GTX103062_40150_20180427_WB_M_testis_19011506_468.jpg
GTX103062_40150_IHC_M_19011506_625.jpg

Cat No. GTX103062

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

38 kDa. ( Note )

Positive Control

293T , HeLa , Molt-4 , Raji , mouse testis

Predict Reactivity

Rat, Zebrafish, Bovine, Japanese Medaka(>80% identity)
PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.9 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DMC1. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

Dna Meiotic Recombinase 1,Dmc1H,Lim15,Dj199H16.1,Dmc1

Cellular Localization

Nucleus

Background

The protein encoded by this gene is essential for meiotic homologous recombination. Genetic recombination in meiosis plays an important role in generating diversity of genetic information. The product of this gene is structurally and evolutionary related to the products of the yeast RAD51 and E. coli RecA genes. Alternative splice variants of this gene have been described but their full-length nature has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX103062_IHC_19011506_513.jpg

GTX103062 IHC-P Image

Immunohistochemical analysis of paraffin-embedded Cal27 xenograft , using DMC1(GTX103062) antibody at 1:500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX103062_WB_19011506_440.jpg

GTX103062 WB Image

Sample (30 ug of whole cell lysate)
A: Molt-4 (GTX27912)
10% SDS PAGE
GTX103062 diluted at 1:1000

GTX103062_40150_20180427_WB_M_testis_19011506_468.jpg

GTX103062 WB Image

Mouse tissue extract (50 µg) was separated by 10% SDS-PAGE, and the membrane was blotted with DMC1 antibody [N1C1] (GTX103062) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

GTX103062_40150_IHC_M_19011506_625.jpg

GTX103062 IHC-P Image

DMC1 antibody [N1C1] detects DMC1 protein at nucleolus on mouse testis by immunohistochemical analysis.
Sample: Paraffin-embedded mouse testis.
DMC1 antibody [N1C1] (GTX103062) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169