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DMP4 antibody

Orthogonal Overexpression
Anti-DMP4 antibody used in Western Blot (WB). GTX110258
Anti-DMP4 antibody used in Western Blot (WB). GTX110258
Anti-DMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX110258
Anti-DMP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110258

Cat. No. GTX110258

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DMP4 products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

66 kDa. ( Note )

Positive Control

U87-MG conditioned medium

Predict Reactivity

Mouse, Rat, Rhesus Monkey(>80% identity)

PROPERTIES

Form

Liquid

Buffer

PBS, 1% BSA, 20% Glycerol

Preservative

0.025% ProClin 300

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.3 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DMP4. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950252

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

FAM20C golgi associated secretory pathway kinase , DMP-4 , DMP4 , G-CK , GEF-CK , RNS

Cellular Localization

Secreted

Background

Calcium-binding protein which may play a role in dentin mineralization.

Database

Research Area

DATA IMAGES

Anti-DMP4 antibody used in Western Blot (WB). GTX110258

GTX110258 WB Image

Overexpression

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DMP4 antibody (GTX110258) diluted at 1:1250. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DMP4 antibody used in Western Blot (WB). GTX110258

GTX110258 WB Image

Orthogonal

U87-MG whole cell extract and conditioned medium (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DMP4 antibody (GTX110258) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DMP4 antibody used in IHC (Paraffin sections) (IHC-P). GTX110258

GTX110258 IHC-P Image

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using DMP4(GTX110258) antibody at 1:100 dilution.
Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Anti-DMP4 antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX110258

GTX110258 ICC/IF Image

Immunofluorescence analysis of methanol-fixed Hep3B, using DMP4(GTX110258) antibody at 1:200 dilution.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 399
$ 169