GeneTex
United States (US)

DMPK antibody

GTX101248_41122_IHC_M_19011506_244.jpg
GTX101248_41122_WB_19011506_575.jpg

Cat No. GTX101248

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IHC-P
Reactivity Human, Mouse
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

69 kDa. ( Note )

Positive Control

U87-MG , SK-N-SH , SK-N-AS

Predict Reactivity

Rhesus Monkey(>80% identity)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1mg/ml(Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the center region of human DMPK. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

MDPK Antibody, DMK Antibody, DM1PK Antibody, MT-PK Antibody, DM1 Antibody, DM Antibody, dystrophia myotonica-protein kinase Antibody

Background

The protein encoded by this gene is a serine-threonine kinase that is closely related to other kinases that interact with members of the Rho family of small GTPases. Substrates for this enzyme include myogenin, the beta-subunit of the L-type calcium channels, and phospholemman. The 3' untranslated region of this gene contains 5-37 copies of a CTG trinucleotide repeat. Expansion of this unstable motif to 50-5,000 copies causes myotonic dystrophy type I, which increases in severity with increasing repeat element copy number. Repeat expansion is associated with condensation of local chromatin structure that disrupts the expression of genes in this region. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined. [provided by RefSeq]

Database

Research Area

DATA IMAGES
GTX101248_41122_IHC_M_19011506_244.jpg

GTX101248 IHC-P Image

DMPK antibody detects DMPK protein at membrane on mouse prostate by immunohistochemical analysis.
Sample: Paraffin-embedded mouse prostate.
DMPK antibody (GTX101248) dilution: 1:500.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

GTX101248_41122_WB_19011506_575.jpg

GTX101248 WB Image

DMPK antibody detects DMPK protein by western blot analysis.
A. 30 μg U87-MG whole cell lysate/extract
B. 30 μg SK-N-SH whole cell lysate/extract
C. 30 μg SK-N-AS whole cell lysate/extract
7.5 % SDS-PAGE
DMPK antibody (GTX101248) dilution: 1:1000

SDS
PBS.pdf
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 319
$ 169