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DNA-PKcs antibody [18-2]

Cat No. GTX21832

Reference 3

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 18-2

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, IP

Reactivity

 Human, Rat
Package
500 μl ($319)
See all DNA-PKcs products

APPLICATION

Application Note

For FuncS: Inhibits (50%) DNA-dependent Phosphorylation - Use at an assay dependent dilution. For IF: Use at an assay dependent dilution. For IHC-P: Use at a dilution of 1:400. For IP: Native and denatured - Use protein G - Use at a dilution of 1:400. For WB: Use at a dilution of 1:200. Optimal dilutions/concentrations should be determined by the researcher.

Calculated MW

469 kDa. ( Note )

Positive Control

LS174T cells, Tonsil

PROPERTIES

Form

Liquid

Buffer

10mM PBS pH7.4, 0.2% BSA

Preservative

0.1% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC. DO NOT FREEZE.

Concentration

0.2 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

It is raised against amino acids 1-2713 of DNA-PK (DNA-dependent protein kinase catalytic subunit) of human origin.

Purification

Protein A affinity purified

Conjugation

Unconjugated

RRID

AB_384700

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Research Area

REFERENCE

Application Reference

Sato H et al. J Virol 2015; 89 (19) : 9709-18 Measles Virus Infection Inactivates Cellular Protein Phosphatase 5 with Consequent Suppression of Sp1 and c-Myc Activities.
Application : WB
Reactivity : Human
You BJ et al. Food Chem Toxicol 2014; 65 : 205-12 Non-homologous end joining pathway is the major route of protection against 4β-hydroxywithanolide E-induced DNA damage in MCF-7 cells.
Application : WB
Reactivity : Human
Chiang JH et al. Hum Exp Toxicol 2013; Effect of DNA damage response by quinazolinone analogue HMJ-38 on human umbilical vein endothelial cells: Evidence for γH2A.X and DNA-PK-dependent pathway.
Application : WB
Reactivity : Human
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SDS
Sodium Azide.pdf
Package List Price ($)
$ 319