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DNA-PKcs antibody [3H6]

Anti-DNA-PKcs antibody [3H6] used in Western Blot (WB). GTX60401
Anti-DNA-PKcs antibody [3H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60401
Anti-DNA-PKcs antibody [3H6] used in ELISA (ELISA). GTX60401
Anti-DNA-PKcs antibody [3H6] used in IHC (Paraffin sections) (IHC-P). GTX60401
Anti-DNA-PKcs antibody [3H6] used in IHC (Paraffin sections) (IHC-P). GTX60401

Cat. No. GTX60401

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 3H6

Isotype

 IgG1

Application

 WB, ICC/IF, IHC-P, ELISA

Reactivity

 Human
Package
100 μl ($409)
See all DNA-PKcs products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1/500 - 1/2000
ICC/IF 1/200 - 1/1000
IHC-P 1/200 - 1/1000
ELISA 1/10000
Not tested in other applications.

Calculated MW

469 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

Ascites

Preservative

0.03% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

Human

Immunogen

Purified recombinant fragment of human PRKDC expressed in E. Coli.

Purification

Unpurified

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus,Nucleolus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-DNA-PKcs antibody [3H6] used in Western Blot (WB). GTX60401

GTX60401 WB Image

WB analysis of HEK293 (1) and DNA-PKcs (AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysate using GTX60401 DNA-PKcs antibody [3H6].

Anti-DNA-PKcs antibody [3H6] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX60401

GTX60401 ICC/IF Image

ICC/IF analysis of HeLa cells using GTX60401 DNA-PKcs antibody [3H6].
Green : DNA-PKcs
Red: Actin filaments

Anti-DNA-PKcs antibody [3H6] used in ELISA (ELISA). GTX60401

GTX60401 ELISA Image

ELISA analysis of antigen using GTX60401 DNA-PKcs antibody [3H6].
Red : Control antigen 100ng
Purple : Antigen 10ng
Green : Antigen 50ng
Blue : Antigen 100ng

Anti-DNA-PKcs antibody [3H6] used in IHC (Paraffin sections) (IHC-P). GTX60401

GTX60401 IHC-P Image

IHC-P analysis of lung cancer (left) and brain tissue (right) using GTX60401 DNA-PKcs antibody [3H6].

Anti-DNA-PKcs antibody [3H6] used in IHC (Paraffin sections) (IHC-P). GTX60401

GTX60401 IHC-P Image

IHC-P analysis of breast cancer (left) and colon cancer (right) using GTX60401 DNA-PKcs antibody [3H6].

REFERENCE

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REVIEW

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SDS
Sodium Azide.pdf
Package List Price ($)
$ 409