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DNA-PKcs antibody [6D1-C11-F10]

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Western Blot (WB). GTX49142
Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunoprecipitation (IP). GTX49142
Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49142

Cat. No. GTX49142

Reference 1

Host

 Mouse

Clonality

 Monoclonal

Clone Name

 6D1-C11-F10

Isotype

 IgG1

Application

 WB, ICC/IF, IP

Reactivity

 Human
Package
100 μg ($399)
See all DNA-PKcs products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

469 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human DNA-PKcs protein fragments expressed in E.coli

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

RRID

AB_2887759

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Western Blot (WB). GTX49142

GTX49142 WB Image

WB analysis of K562 and HeLa lysates using DNA-PKcs antibody at a dilution of 1:1,000.

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunoprecipitation (IP). GTX49142

GTX49142 IP Image

IP analysis of HeLa lysate using control IgG and DNA-PKcs antibody. The precipitates were detected by the same DNA-PKcs antibody.

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49142

GTX49142 ICC/IF Image

ICC/IF analysis of Hela cells using DNA-PKcs antibody at a dilution of 1:100.

REFERENCE

Application Reference

Chakraborty A et al. Nat Commun 2016; 7 : 13049 Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 399