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DNA-PKcs antibody [6D1-C11-F10]

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Western Blot (WB). GTX49142
Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunoprecipitation (IP). GTX49142
Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49142

Cat No. GTX49142

Host

Mouse

Clonality

Monoclonal

Clone Name

6D1-C11-F10

Isotype

IgG1

Application

WB, ICC/IF, IP

Reactivity

Human
Package
100 μg ($339)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB Assay dependent
ICC/IF Assay dependent
IP Assay dependent
Not tested in other applications.

Calculated MW

469 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS pH7.4, 50% Glycerol, 0.1% BSA

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant human DNA-PKcs protein fragments expressed in E.coli

Purification

Affinity purified

Conjugation

Unconjugated

RRID

AB_2887759

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Western Blot (WB). GTX49142

GTX49142 WB Image

WB analysis of K562 and HeLa lysates using DNA-PKcs antibody at a dilution of 1:1,000.

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunoprecipitation (IP). GTX49142

GTX49142 IP Image

IP analysis of HeLa lysate using control IgG and DNA-PKcs antibody. The precipitates were detected by the same DNA-PKcs antibody.

Anti-DNA-PKcs antibody [6D1-C11-F10] used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX49142

GTX49142 ICC/IF Image

ICC/IF analysis of Hela cells using DNA-PKcs antibody at a dilution of 1:100.

REFERENCE

REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf
Package List Price ($)
$ 339