DNA-PKcs antibody [8D3-B12-F11]

Cat. No. GTX49138

Host	Mouse
Clonality	Monoclonal
Clone Name	8D3-B12-F11
Isotype	IgG2b
Application	WB, ICC/IF, IP
Reactivity	Human
Package	100 μg ($399)

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Datasheet
Document

View product citations for antibody GTX49138 on CiteAb

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Recommended Dilution
WB	Assay dependent
ICC/IF	Assay dependent
IP	Assay dependent

Not tested in other applications.

Calculated MW

469 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

PBS, 0.5% BSA, 50% glycerol

Preservative

0.02% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

Batch dependent (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Purified recombinant DNA-PKcs protein fragments expressed in E.coli

Purification

Purified by affinity chromatography

Conjugation

Unconjugated

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus , Nucleolus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Gene ID: 5591 PRKDC
UniProt: P78527 PRKDC

Research Area

DATA IMAGES

GTX49138 IP Image

IP analysis of HeLa lysates using control IgG and DNA-PKcs antibody. The precipitates were detected by the same DNA-PKcs antibody.

GTX49138 ICC/IF Image

ICC/IF analysis of HeLa cells using DNA-PKcs antibody at a dilution of 1:200.

GTX49138 WB Image

WB analysis of Molt-4, HL60, K562 and HeLa lysates using DNA-PKcs antibody at a dilution of 1:1,000.

REFERENCE

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REVIEW

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SDS
PBS.pdf
Glycerol.pdf
Sodium Azide.pdf

Package

List Price ($)

100 μg

$ 399

Qty