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DNA-PKcs antibody [C3], C-term

Anti-DNA-PKcs antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109673
Anti-DNA-PKcs antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109673
Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673
Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673

Cat No. GTX109673

Reference 1

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB, ICC/IF, IHC-P

Reactivity

 Human
Package
100 μl ($329),
25 μl ($169)
See all DNA-PKcs products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
IHC-P 1:100-1:1000
Not tested in other applications.

Calculated MW

469 kDa. ( Note )

Positive Control

293T , Molt-4 , NCI-H929

PROPERTIES

Form

Liquid

Buffer

0.1M Tris-Glycine (pH7), 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human DNA-PKcs. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1951440

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus

Background

The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.[supplied by OMIM]

Database

Research Area

DATA IMAGES

Anti-DNA-PKcs antibody [C3], C-term used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX109673

GTX109673 ICC/IF Image

DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA-PKcs protein stained by DNA-PKcs antibody [C3], C-term (GTX109673) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Scale bar = 10 μm.

Anti-DNA-PKcs antibody [C3], C-term used in IHC (Paraffin sections) (IHC-P). GTX109673

GTX109673 IHC-P Image

DNA-PKcs antibody [C3], C-term detects DNA-PKcs protein at cytosol and nucleus on human ovarian carcinoma by immunohistochemical analysis.
Sample: DNA-PKcs antibody [C3], C-term (GTX109673) dilution: 1:250.

Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673

GTX109673 WB Image

Sample(30 ug whole cell lysate)
A:293T
B:MOLT4 (GTX27912)
5% SDS PAGE
GTX109673 diluted at 1:1000

Anti-DNA-PKcs antibody [C3], C-term used in Western Blot (WB). GTX109673

GTX109673 WB Image

DNA PKcs antibody detects DNA PKcs protein by western blot analysis. Whole cell extracts (30 μg) was separated by 5% SDS-PAGE, and the membrane was blotted with DNA PKcs antibody (GTX109673) at a dilution of 1:500.

REFERENCE

Application Reference

Jang K et al. Genome Biol 2020; 21 (1) : 155 Computational inference of cancer-specific vulnerabilities in clinical samples.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 329
$ 169