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DNA-PKcs (phospho Ser2056) antibody

Anti-DNA-PKcs (phospho Ser2056) antibody used in Western Blot (WB). GTX132793
Anti-DNA-PKcs (phospho Ser2056) antibody used in Western Blot (WB). GTX132793
Anti-DNA-PKcs (phospho Ser2056) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132793

Cat No. GTX132793

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, ICC/IF
Reactivity Human
Package
100 μl ($319),
25 μl ($169)
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF 1:100-1:1000
Not tested in other applications.

Calculated MW

469 kDa. ( Note )

Positive Control

293T , 293T (100 J/m2 UVC treatment)
PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.68 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human DNA-PKcs (phospho Ser2056). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit,DNA-PKC,DNA-PKcs,DNAPK,DNAPKc,DNPK1,HYRC,HYRC1,IMD26,XRCC7,p350

Cellular Localization

Nucleus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Research Area

DATA IMAGES
Anti-DNA-PKcs (phospho Ser2056) antibody used in Western Blot (WB). GTX132793

GTX132793 WB Image

Non-transfected (–) and transfected (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (GTX132793) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Anti-DNA-PKcs (phospho Ser2056) antibody used in Western Blot (WB). GTX132793

GTX132793 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (GTX132793) diluted at 1:1000.

Anti-DNA-PKcs (phospho Ser2056) antibody used in Immunocytochemistry/ Immunofluorescence (ICC/IF). GTX132793

GTX132793 ICC/IF Image

DNA-PKcs (phospho Ser2056) antibody detects DNA-PKcs (phospho Ser2056) protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA-PKcs (phospho Ser2056) stained by DNA-PKcs (phospho Ser2056) antibody (GTX132793) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar= 10 μm.

REFERENCE
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REVIEW
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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf
Package List Price ($)
$ 319
$ 169