DNA-PKcs (phospho Ser2056) antibody

Cat No. GTX132794

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF
Reactivity	Human
Package	100 μl ($319), 25 μl ($169)

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Datasheet
Document

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500-1:3000
ICC/IF	1:100-1:1000

Not tested in other applications.

Calculated MW

469 kDa. ( Note )

Positive Control

293T , 293T (100 J/m2 UVC treatment)

PROPERTIES

Form

Liquid

Buffer

1XPBS, 1% BSA, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative.

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.6 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Carrier-protein conjugated synthetic peptide corresponding to residues around human DNA-PKcs (phospho Ser2056). The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit,DNA-PKC,DNA-PKcs,DNAPK,DNAPKc,DNPK1,HYRC,HYRC1,IMD26,XRCC7,p350

Cellular Localization

Nucleus

Background

This gene encodes the catalytic subunit of the DNA-dependent protein kinase (DNA-PK). It functions with the Ku70/Ku80 heterodimer protein in DNA double strand break repair and recombination. The protein encoded is a member of the PI3/PI4-kinase family.[provided by RefSeq, Jul 2010]

Database

Gene ID: 5591 PRKDC
UniProt: P78527 PRKDC

Research Area

DATA IMAGES

GTX132794 ICC/IF Image

DNA-PKcs (phospho Ser2056) antibody detects DNA-PKcs (phospho Ser2056) protein at nucleus by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: DNA-PKcs (phospho Ser2056) stained by DNA-PKcs (phospho Ser2056) antibody (GTX132794) diluted at 1:500.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (GTX628802) diluted at 1:500.
Scale bar= 10 μm.

GTX132794 WB Image

Untreated (–) and treated (+) 293T whole cell extracts (60 µg) were separated by 5% SDS-PAGE, and the membrane was blotted with DNA-PKcs (phospho Ser2056) antibody (GTX132794) diluted at 1:500.

REFERENCE

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SDS
PBS.pdf
Glycerol.pdf
Proclin.pdf

Package

List Price ($)

100 μl

$ 319

25 μl

$ 169

Qty