DNA-PKcs (phospho Thr2609) antibody

Name: DNA-PKcs (phospho Thr2609) antibody
Brand: GeneTex
SKU: GTX24194
Price: 319.00 USD
Rating: 5 (1 reviews)

Anti-DNA-PKcs (phospho Thr2609) antibody used in Western Blot (WB). GTX24194

Cat No. GTX24194

Reference 4

Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Application	WB, ICC/IF, ELISA
Reactivity	Human, Chimpanzee
Package	50 μg ($319)

See all DNA-PKcs products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.

Application	Dilution
WB	1:500 - 1:2000
ICC/IF	Assay dependent
ELISA	1:300000
Note : WB Alternate splice variants have been reported. Recommed performing IP prior to WB.

Not tested in other applications.

Calculated MW

469 kDa. ( Note )

PROPERTIES

Form

Liquid

Buffer

0.02M Potassium Phosphate pH7.2, 0.15M Sodium Chloride

Preservative

0.01% Sodium azide

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

0.93 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Synthetic peptide - amino acids 2604-2614 of human DNA PKcs - phosphorylated at the pT2609 residue

Purification

Purified by antigen-affinity chromatography.
Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. The product is sterile filtered.

Conjugation

Unconjugated

RRID

AB_370110

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

protein kinase, DNA-activated, catalytic subunit , DNA-PKC , DNA-PKcs , DNAPK , DNAPKc , DNPK1 , HYRC , HYRC1 , IMD26 , XRCC7 , p350

Cellular Localization

Nucleus,Nucleolus

Background

DNA PK is the key component of the non-homologous end-joining (NHEJ) pathway of DSB repair in mammalian cells. DNA PK consists of a heterodimeric DNA-binding subunit (Ku70/80) and an approximately 465 kDa catalytic subunit (DNA PKcs). DNA PKcs is a serine/threonine protein kinase whose activity is greatly stimulated by its recruitment to DNA breaks by the Ku heterodimer.

Database

Gene ID: 5591 PRKDC
UniProt: P78527 PRKDC

Research Area

DATA IMAGES

GTX24194 WB Image

Western blot using GeneTex Affinity Purified anti-DNAPKcs antibody (GTX24194) shows detection of a 460 kDa band corresponding to human DNAPKcs in various preparations. Lane 1: Fus1 untreated Lane 2: Fus1 IR (20Gy, 4h) Lane 3: Fus1 DNAPK inhibitor + IR Lane 4: MO59J (DNAPK-) untreated Lane 5: MO59J IR Lane 6: Fus1 untreated Lane 7: Fus1 IR (20Gy, 4h) Lane 8: Fus1 DNAPK inhibitor + IR Lane 9: MO59J untreated Lane 10: MO59J IR. Lanes 1-5 are nuclear extract whereas lanes 6-10 are whole cell lysates. MO59J is a cell line that lacks DNA-PKcs. FUS1 is the matched cell line complemented with a chromosomal fragment containing the DNA-PKcs gene. Approximately 20 μg of lysate was run on SDS-PAGE and transferred onto nitrocellulose, followed by reaction with a 1:1,000 dilution of anti-DNAPKcs antibody. Detection occurred using a 1:5,000 dilution of HRP-labeled Goat anti-Rabbit IgG for 1 hour at room temperature. A chemiluminescence system was used for signal detection (Roche) using a 1 min exposure time.

REFERENCE

Application Reference

Nixon BR et al. FASEB J 2018; : fj201800336R Nuclear localized Raf1 isoform alters DNA-dependent protein kinase activity and the DNA damage response.

Application : WB

Reactivity : Human

Jarboe JS et al. Radiother Oncol 2012; 103 (3) : 380-7 Kinomic profiling approach identifies Trk as a novel radiation modulator.

Jarboe JS et al. Clin Cancer Res 2012; 18 (11) : 3030-41 MARCKS regulates growth and radiation sensitivity and is a novel prognostic factor for glioma.

Nowsheen S et al. PLoS One 2011; 6 (8) : e24148 Cetuximab augments cytotoxicity with poly (adp-ribose) polymerase inhibition in head and neck cancer.

Application : ICC/IF

Reactivity : Human

Submit a Reference

REVIEW