Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with anti-DNA polymerase 1 antibody 1. Purified DNA polymerase 1 ( 20 ng), WB 2. Purified DNA polymerase 1 (10 ng), WB 3. Crude lysate of E. coli strain AB1157 (10 μg), WB 4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP for IP was used.

Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using anti-DNA polymerase 1 antibody. 1; Purified E. coli DNA polymerase 1 (10 ng) 2; Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1/2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.