GeneTex
United States (US)

DNA Polymerase I (E coli) antibody

GTX64108_20170328_IP_18121411_117.jpg
GTX64108_20170328_WB_18121411_228.jpg

Cat No. GTX64108

Host Rabbit
Clonality Polyclonal
Isotype IgG
Application WB, IP
Reactivity E. coli
APPLICATION

Application Note

Western blot. (1:1,000-1:5,000 dilution)
PROPERTIES

Form

Liquid

Buffer

Antiserum added with sodium azide 0.05%

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Antigen Species

E. coli

Immunogen

Full-size recombinant E. coli DNA polymerase 1.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET
DATA IMAGES
GTX64108_20170328_IP_18121411_117.jpg

GTX64108 IP Image

Immunoprecipitation of DNA polymerase 1 from crude lysate of E. coli with anti-DNA polymerase 1 antibody 1. Purified DNA polymerase 1 ( 20 ng), WB 2. Purified DNA polymerase 1 (10 ng), WB 3. Crude lysate of E. coli strain AB1157 (10 μg), WB 4. The crude lysate of E.coli cells was reacted with anti-DNA polymerase 1 antibody and precipitated with protein G conjugated magnetic beads, and analyzed by WB by using anti-DNA polymerase 1 antibody. As the secondary antibody, anti-rabbit IgG antibody conjugated with HRP for IP was used.

GTX64108_20170328_WB_18121411_228.jpg

GTX64108 WB Image

Western blot analysis of DNA polymerase 1 in crude extract of E. coli cells by using anti-DNA polymerase 1 antibody. 1; Purified E. coli DNA polymerase 1 (10 ng) 2; Sonic lysate supernatant of E. coli strain AB1157 cells. Primary antibody was used at 1/2,000 dilution. Predicted molecular mass of DNA polymerase 1 is 103 kDa.

Package List Price ($)
$ 349