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DNA ligase I antibody [C2C3], C-term

Anti-DNA ligase I antibody [C2C3], C-term used in Western Blot (WB). GTX102936

Cat. No. GTX102936

Reference 2

Host

 Rabbit

Clonality

 Polyclonal

Isotype

 IgG

Application

 WB

Reactivity

 Human
Package
100 μl ($399),
25 μl ($169)
See all DNA ligase I products

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

A431 , H1299

Predict Reactivity

Mouse, Rat, Bovine(>80% identity)

PROPERTIES

Form

Liquid

Buffer

0.1M Tris, 0.1M Glycine, 10% Glycerol

Preservative

0.01% Thimerosal

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human DNA ligase I. The exact sequence is proprietary.

Purification

Purified by antigen-affinity chromatography.

Conjugation

Unconjugated

RRID

AB_1950811

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 1

Cellular Localization

Nucleus

Background

LIG1 encodes DNA ligase I, with functions in DNA replication and the base excision repair process. Mutations in LIG1 that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. [provided by RefSeq]

Database

Research Area

DATA IMAGES

Anti-DNA ligase I antibody [C2C3], C-term used in Western Blot (WB). GTX102936

GTX102936 WB Image

Sample (30 ug of whole cell lysate)
A: A431 (GTX27909)
B: H1299
7.5% SDS PAGE
GTX102936 diluted at 1:1000

REFERENCE

Application Reference

Liddiard K et al. Nucleic Acids Res 2018; (Epub) DNA Ligase 1 is an essential mediator of sister chromatid telomere fusions in G2 cell cycle phase.
Application : WB
Reactivity : Human
Valdez BC et al. Leuk Lymphoma 2017; : 43112 the parp inhibitor olaparib enhances the cytotoxicity of combined *, * and * in lymphoma cells.
Application : WB
Reactivity : Human
Submit a Reference

REVIEW

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SDS
Glycerol.pdf
Thimerosal.pdf
Package List Price ($)
$ 399
$ 169