GeneTex
United States (US)

DNA ligase I antibody, Internal (near C-Terminus)

GTX88393_2379_WB_18122102_915.jpg

Cat No. GTX88393

Host Goat
Clonality Polyclonal
Isotype IgG
Application WB
Reactivity Human
APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 0.1-0.3μg/ml
Not tested in other applications.

Calculated MW

102 kDa. ( Note )

Positive Control

The peptide used to generate this antibody is available for purchase (GTX88393-PEP).

Predict Reactivity

Rat(>80% identity)
PROPERTIES

Form

Liquid

Buffer

Tris saline pH7.3, 0.02% sodium azide, 0.5% BSA.

Concentration

0.50mg/ml

Antigen Species

Human

Immunogen

Peptide with sequence C-RVREDKQPEQATTS, from the internal region (near C-Terminus) of the protein sequence according to NP_000225.1.

Purification

Purified by ammonium sulphate precipitation followed by antigen affinity chromatography

Conjugation

Unconjugated

Note

For In vitro laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.
TARGET

Synonyms

LIG1 Antibody

Background

This gene encodes a member of the ATP-dependent DNA ligase protein family. The encoded protein functions in DNA replication, recombination, and the base excision repair process. Mutations in this gene that lead to DNA ligase I deficiency result in immunodeficiency and increased sensitivity to DNA-damaging agents. Disruption of this gene may also be associated with a variety of cancers. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
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Database

Research Area

DATA IMAGES
GTX88393_2379_WB_18122102_915.jpg

GTX88393 WB Image

WB analysis of HeLa nuclear lysate using GTX88393 DNA ligase I antibody, Internal (near C-Terminus).
Dilution : 0.1µg/ml
Loading : 35µg protein in RIPA buffer