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DNA ligase III antibody [1F3]

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

Cat. No. GTX70143

Host

Mouse

Clonality

Monoclonal

Clone Name

1F3

Isotype

IgG1

Application

WB, ICC/IF, IP, Blocking, PLA

Reactivity

Human, Mouse, Chicken
Package
100 μl ($399)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Recommended Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Blocking Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

113 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Preservative

No preservative

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human DNA Ligase III-b.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

RRID

AB_372134

Note

For laboratory research use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

Purchasers shall not, and agree not to enable third parties to, analyze, copy, reverse engineer or otherwise attempt to determine the structure or sequence of the product.

TARGET

Synonyms

DNA ligase 3 , LIG2 , LIG3alpha

Background

This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]

Database

Research Area

DATA IMAGES

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX70143) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (GTX70143) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The indicated band with observed MW (103 kD) may represent the larger isoform of human DNA Ligase III as reported by Chen J et al and Nash RA et al.

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

The data was published in the journal PLoS One in 2013. PMID: 23555685

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 399
Custom Conjugations