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DNA ligase III antibody [1F3]

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143
Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

Cat No. GTX70143

Host

Mouse

Clonality

Monoclonal

Clone Name

1F3

Isotype

IgG1

Application

WB, ICC/IF, IP, Blocking, PLA

Reactivity

Human, Mouse, Chicken
Package
100 μl ($329)

APPLICATION

Application Note

*Optimal dilutions/concentrations should be determined by the researcher.
Application Dilution
WB 1:500-1:3000
ICC/IF Assay dependent
IP Assay dependent
Blocking Assay dependent
PLA Assay dependent
Not tested in other applications.

Calculated MW

100 kDa. ( Note )

Positive Control

293T , A431 , HeLa , HepG2

PROPERTIES

Form

Liquid

Buffer

PBS

Storage

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.

Concentration

1 mg/ml (Please refer to the vial label for the specific concentration.)

Antigen Species

Human

Immunogen

Recombinant full-length human DNA Ligase III-b.

Purification

Protein G affinity purified.
From hybridoma culture supernatant.

Conjugation

Unconjugated

Note

For laboratory use only. Not for any clinical, therapeutic, or diagnostic use in humans or animals. Not for animal or human consumption.

TARGET

Synonyms

Dna Ligase 3 , Lig2 , Lig3Alpha , Lig3

Cellular Localization

Nuclear and Mitochondrial

Background

There are four distinct ligase proteins found in mammalian cells. The activity of these DNA ligases is required for the joining of DNA strand breaks that occur during DNA replication, repair and recombination. DNA Ligase III gene product migrates as a 100kDa (a-form) protein in somatic tissues, is ubiquitously expressed and complexes with XRCC-1 and a 97kDa (§-form) protein that is found in testis. DNA Ligase III is involved in rejoining DNA single-strand breaks and in the base excision repair pathway.

Database

Research Area

DATA IMAGES

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

The WB analysis of DNA ligase III antibody was published by Paul K and colleagues in the journal PLoS One in 2013.PMID: 23555685

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

DNA ligase III antibody detects DNA ligase III protein by western blot analysis. Whole cell extracts (30 μg) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNA ligase III antibody (GTX70143) diluted by 1:500. The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.

Anti-DNA ligase III antibody [1F3] used in Western Blot (WB). GTX70143

GTX70143 WB Image

DNA ligase III detects DNA ligase III antibody [1F3] protein by western blot analysis.
A. 30 μg 293T whole cell lysate/extract
B. 30 μg A431 whole cell lysate/extract
C. 30 μg HeLa whole cell lysate/extract
D. 30 μg HepG2 whole cell lysate/extract
7.5% SDS-PAGE
DNA ligase III (GTX70143) dilution: 1:500
The HRP-conjugated anti-mouse IgG antibody (GTX213111-01) was used to detect the primary antibody.
The indicated band with observed MW (103 kD) may represent the larger isoform of human DNA Ligase III as reported by Chen J et al and Nash RA et al.

REFERENCE

REVIEW

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Application Reference
SDS
PBS.pdf
Package List Price ($)
$ 329